Bacterial lactate dehydrogenases

Garvie, Ellen I.

doi:10.1128/mr.44.1.106-139.1980

Cited by 307 publications

(91 citation statements)

References 98 publications

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“…In the presence of 10 mM fructose 1,6-bisphosphate, V max increased to 450 mmol min 21 . This activation of Ldh by fructose 1,6-bisphosphate has been described for the enzymes of a great number of Gram-positive bacteria (Garvie, 1980) and was not specific to the C. glutamicum enzyme.…”

Section: Resultsmentioning

confidence: 59%

“…Both fructose 1,6-bisphosphate and a high NADH/NAD ratio have been described as activators of Ldh activity (Garvie, 1980;Garrigues et al, 1997). Increase in ldh expression and Ldh activation then allowed the efficient conversion of pyruvate into lactate and a progressive decrease of the intracellular concentration of glycolytic intermediates and the NADH/NAD ratio.…”

Section: Time-course Of Expression Of Ldhmentioning

confidence: 99%

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Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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Corynebacterium glutamicum is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. In an effort to understand C. glutamicum metabolism under biotin limitation, growth of the type strain ATCC 13032 was investigated in batch cultures and a time-course analysis was performed. A transient excretion of organic acids was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the ldh-encoded l-lactate dehydrogenase (Ldh). Features of Ldh activity and ldh transcription were analysed. The ldh gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcriptional repressor also acting on most phosphotransferase system (PTS) genes. Electrophoretic mobility shift assays (EMSAs) and site-directed mutagenesis allowed the identification of the SugR-binding site. Effector studies using EMSAs and analysis of ldh expression in a ptsF mutant revealed fructose 1-phosphate as a highly efficient negative effector of SugR. Fructose 1,6-bisphosphate also affected SugR binding.

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Section: Resultsmentioning

confidence: 59%

Section: Time-course Of Expression Of Ldhmentioning

confidence: 99%

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

et al. 2009

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“…Supplementation of Cu (CuSO 4 or Cu 2 O) in broilers' diet compared with the non-supplemented diet suppressed the abundance of Streptococcaceae . The genera Streptococcus is active in the process of simple sugar fermentation into lactate ( Garvie, 1980 ; Zoetendal et al., 2012 ). Whereas, some species of the genus (e.g., Streptococcus bovis ) are considered as major opportunistic pathogens, which can result in many diseases ( Abdulamir et al., 2011 ; Munita et al., 2012 ; Qiao et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…Lactobacillaceae ( Lactobacillus spp. ) is one of the main lactic acid-producing bacteria, and the primary end product of these bacteria is lactic acid ( Garvie, 1980 ; Rajilić-Stojanović and de Vos, 2014 ) which has positive effects on growth. Interestingly, in a study by Gharib-Naseri et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers

et al. 2021

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An experiment was conducted to determine the effects of two sources of copper ( Cu ) from copper sulfate ( CuSO 4 ) and dicopper oxide ( Cu 2 O , CoRouge) at three levels of inclusion (15, 75, and 150 mg/kg) on growth performance and gut microbiota of broilers. A total of 840 one-d-old male chickens (Ross 308) were weighed and randomly allocated to seven dietary treatments: negative control ( NC , a basal diet without Cu addition), and the NC supplemented with 15, 75, or 150 mg Cu/kg from CuSO 4 or Cu 2 O (12 replicate pens/treatment, 10 chicks per pen). Broilers were challenged by reusing an old litter with high concentrations in Clostridium perfringens to promote necrotic enteritis. Broiler performance was registered at d 21, 35, and 42. Excreta samples were collected at d 14, 28, and 42 for antimicrobial resistance ( AMR ) analyses. At d 43, one broiler per pen was euthanized to obtain ileal content for microbial characterization. Body weight d 35 and daily gain d 42 improved ( P < 0.05) in Cu 2 O as Cu dose inclusion increased from 15 mg/kg to 150 mg/kg. Supplementation of 150 mg/kg of Cu from Cu 2 O decreased the abundance ( P < 0.01) of some families such as Streptococcaceae and Corynebacteriaceae and increased the abundance ( P < 0.05) of some commensal bacteria like Clostridiaceae and Peptostreptococcaceae. Phenotypic AMR was not different among treatments on d 14 and 28. Isolated Enterococcus spp. from broilers fed the NC diet on d 42 showed higher ( P < 0.05) resistance to enrofloxacin, gentamicin, and chloramphenicol compared with Cu treatments. By contrast, the isolated Escherichia coli from broilers fed 150 mg/kg of Cu, either from CuSO 4 or Cu 2 O, showed higher ( P < 0.05) resistance to streptomycin and chloramphenicol compared to the NC. This study suggests that supplementing 150 mg/kg of Cu from Cu 2 O establishes changes in the gut microbiota by regulating the bacterial population in the ileum, which may explain the positive impact on broilers' growth performance.

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“…Καηά ημ ιεηααμθζζιυ ηςκ ζαηπάνςκ ιέζς ηδξ μδμφ Embden-Meyerhof, ηα μλοβαθαηηζηά ααηηήνζα αλζμπμζμφκ ημ πονμζηαθοθζηυ μλφ ηαζ ημ ιεηαηνέπμοκ ζε βαθαηηζηυ ιε ακηζδνάζεζξ πμο ηαηαθφμκηαζ απυ ηα έκγοια L-βαθαηηζηή ηαζ D-βαθαηηζηή δετδνμβμκάζδ. Ο ηφπμξ ημο ζζμιενμφξ ημο βαθαηηζημφ μλέμξ πμο πνμηφπηεζ, ελανηάηαζ απυ ημ βέκμξ ηαζ ημ είδμξ ημο ιζηνμμνβακζζιμφ πμο πνδζζιμπμζείηαζ, ημο μπμίμο ημ έκγοιμ ζοιιεηέπεζ ζηδ δζαδζηαζία ηδξ παναβςβήξ ημο μλέμξ (Garvie, 1980, Axelsson, 1998. Σμ Lβαθαηηζηυ μλφ πανάβεηαζ απυ ηα βέκδ ηςκ ιζηνμμνβακζζιχκ Aerococcus, Carnobacterium, Enterococcus, Lactococcus, Tetragenococcus ηαζ Vagococcus, εκχ ημ D-απυ ηα βέκδ Leuconostoc ηαζ Oenococcus.…”

Section: γαθαηηζηό μλύunclassified

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ABC system ATP-Binding Cassette, ηαζέηα δέζιεοζδξ ATP ALS α-acetolactate synthase, α-μλοβαθαηηζηή ζοκεεηάζδ AMPs antimicrobial peptides, ακηζιζηνμαζαηά πεπηίδζα APS ammonium persulfate, οπενεεζζηυ αιιχκζμ AU arbitrary units of activity, ιμκάδεξ εκενβυηδηαξ BN PAGE blue native polyacrylamide gel electrophoresis C+G cytocine+guanine, ηοημζίκδ+βμοακίκδ CcpA carbon catabolite protein A, πνςηεΐκδ Α ηαηααμθζζιμφ ημο άκεναηα CDW cell dry weight, λδνή αζμιάγα (g/L) CI creaming index, δείηηδξ ηνειμπμίδζδξ D-GPT D-glutamate pyruvate transaminase, D-βθμοηαιζηή-πονμζηαθοθζηή ηνακζαιζκάζδ DOT dissolved oxygen tension, ιενζηή πίεζδ μλοβυκμο (%) EMP Embden-Meyerhof-Parnas pathway, μδυξ Embden-Meyerhof-Parnas ESI MS electrospray ionization, ζμκηζζιυξ ιε δθεηηνμρεηαζιυ FBP fructose bisphosphate, δζθςζθμνζηή θνμοηηυγδ FWP formic acid/water/2-propanol, ιονιδηζηυ μλφ/κενυ/πνμπακυθδ GAPDH glyceraldehyde phosphate dehydrogenase, αθοδνμβμκάζδ ηδξ θςζθμνζηήξ βθοηεναθδεΰδδξ LDH lactate dehydrogenase, βαθαηηζηή αθοδνμβμκάζδ LPS lactoperoxidase system, ζφζηδια θαηημπενμλεζδάζδξ MALDI MS matrix-assisted laser desorption/ionization, ζμκηζζιυξ εηνυθδζδξ ιε ηδ αμήεεζα οθζημφ ιήηναξ MS mass spectrometry, θαζιαημιεηνία ιάγαξ MSM meat simulation medium, οπυζηνςια πνμζμιμίςζδξ ηνέαημξ m/z θυβμξ ιάγαξ πνμξ θμνηίμ (-) NICE Νisin Controlled Expression System, ζφζηδια εθεβπυιεκδξ έηθναζδξ κζζίκδξ NOX NADH oxidase, NADH μλεζδάζδ PAGE polyacrylamide gel electrophoresis, δθεηηνμθυνδζδ ζε πήηηςια πμθοαηνοθαιζδίμο PDH pyruvate dehydrogenase, πονμζηαθοθζηή δετδνμβμκάζδ PEF pulse electric field, παθθυιεκμ δθεηηνζηυ πεδίμ PEP:PTS phosphoenolo-pyruvate:phosphotransferase system, ζφζηδια θςζθμεκμθμπονμζηαθοθζημφ:θςζθμηνακζθενάζδξ PFK phosphofructokinase, θςζθμθνμοηημηζκάζδ PFL pyruvate formate lyase, θοάζδ ιονιδηζημφ μλέμξ PMF proton motif force, πνςημκζεβενηζηή δφκαιδ PYK pyruvate kinase, πονμζηαθοθζηή ηζκάζδ q p specific production rate, εζδζηή ηαπφηδηα παναβςβήξ ααηηδνζμζίκδξ (AU/g/h) αηνζαήξ πνμζδζμνζζιυξ ημο ιμνζαημφ αάνμοξ ηδξ ηαζ ηδξ αθθδθμοπίαξ ημο αιζκμηεθζημφ άηνμο ημο ιμνίμο ηδξ .

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Bacterial lactate dehydrogenases

Cited by 307 publications

References 98 publications

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum

Effects of dicopper oxide and copper sulfate on growth performance and gut microbiota in broilers

Διερεύνηση Των Συνθηκών Παραγωγής Βακτηριοσινών Από Στελέχη Οξυγαλακτικών Βακτηρίων Που Απομονώθηκαν Από Αλλαντικά Αέρος Και Μελέτη Των Ιδιοτήτων Τους

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