Diare merupakan buang air besar sebanyak tiga kali atau lebih dalam sehari dengan konsistensi feses cair, baik disertai darah dan atau lendir. Diare umumnya disebabkan oleh beberapa mikroba, salah satunya yaitu Escherichia coli. Kayu manis adalah tanaman yang diketahui memiliki khasiat sebagai obat. Kayu manis memiliki aktivitas sebagai antimikroba, karena mengandung senyawa aktif seperti minyak atsiri. Penelitian ini bertujuan untuk menganalisis aktivitas antibakteri ekstrak kayu manis terhadap pertumbuhan Escherichia coli. Metode penelitian menggunakan design penelitian post-test only with control group dengan metode difusi kertas cakram pada konsentrasi 25%, 50%, 75% dan 100%. Hasil menunjukkan bahwa ekstrak kayu manis konsentrasi 100% dan 75% memiliki aktivitas dalam menghambat pertumbuhan Escherichia coli dengan diameter zona hambat 4.85mm dan 3.15mm.
Phosphorus (P) and potassium (K) are essential for the growth and development of plants. Most of phosphate and potassium are bound in the form of rocks P or K-minerals which not be directly absorbed by plants. The utilization of microbes including Phosphate Solubilizing Bacteria (PSB) and Potassium Solubilizing Bacteria (KSB) is deemed as an alternative to address the issue of P and K availability. The objective of the research was to select and identify the phosphate-potassium solubilizing bacteria and measure the quantitative estimation of solubilized P and K. Phosphatepotassium solubilizing bacteria were collected and regrown using dot method on Pikovskaya solid medium to solubilize P and on Aleksandrov solid medium to solubilize K, incubated for 7 days at room temperature. The QC3.a.1 and QC3.d.5 showed the highest solubilization index on Pikovskaya medium containing Ca 3 (PO 4 ) 2 , while QC3.a.2 produced the highest amount of soluble phosphate. The QC3.a.2 showed the best of potassium solubilizing activity based on clear zone formation, while QC3.a.1 had the best ability in feldspar hydrolyzation. The three strains were Gram-negative bacteria. Phylogenetic analysis based on 16S rRNA gene sequence showed that isolate QC3.a.1 was closely related to Burkholderia, isolate QC3.a.2 was closely related to Serratia and isolate QC3.d.5 was closely related to Pseudomonas putida.
Escherichia coli (E. coli) dan Shigella dysenteriae (S. dysenteriae) merupakan bakteri penyebab diare. Salah satu tumbuhan yang dapat digunakan sebagai pengobatan untuk diare adalah daun pepaya. Daun papaya memiliki kandungan senyawa aktif seperti papain, tanin, alkaloid, flavonoid, fenol, saponin, dan steroid yang diduga memiliki efek antimikroba. Penelitian ini bertujuan untuk menganalisis efektivitas pemberian ekstrak daun pepaya (Carica papaya L.) dengan berbagai konsentrasi terhadap pertumbuhan bakteri E. coli dan S. dysenteriae. Penelitian ini menggunakan rancangan post-test only with control group dengan metode difusi kertas cakram (Kirby-Bauer). Zona hambat yang terbentuk diukur menggunakan jangka sorong dan dianalisis menggunakan uji One Way ANOVA. Hasil penelitian menunjukkan bahwa ekstrak daun papaya dapat menghambat pertumbuhan bakteri E. coli dan S. dysenteriae secara in-vitro. Analisis uji statistika didapatkan (p<0,05) yang artinya terdapat perbedaan nyata antar kelompok perlakuan setelah pemberian ekstrak daun pepaya terhadap pertumbuhan bakteri E. coli dan S. dysenteriae.
Acute diarrhea is liquid stool with a frequency of more than 3 times a day and lasts less than 14 days. One of the most common causes of acute diarrhea is Escherichia coli. Diarrhea caused by bacteria can be treated with the use of antibiotics, but the relatively high intensity of antibiotic use causes various health problems, especially bacterial resistance. Therefore, the search for antibacterial agents derived from natural ingredients was carried out as an alternative treatment. Bay leaf (Syzygium polyanthum) is one of the natural ingredients that has the potential as an antibacterial because it contains active compounds such as essential oils, flavonoids (quercetin), saponins, tannins, and alkaloids. This study aimed to analyze the antibacterial activity of the ethanolic extract of bay leaves on the growth of Escherichia coli. This research uses the dilution method which aims to determine the Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC). The results showed that the MIC value at a concentration of 200mg/ml and the MBC value at a concentration of 500mg/ml.
INTRODUCTION : Inbalance of microbiota in gastrointestinal tract have the risk of getting gastrointestinal infections, one of them is diarrhea. AIM : The aim of this study was to determine bifidobacterium and eschericia coli microbiota and compare the microbiota obtained between exclusive breast fed and formula fed Indonesian infants village andalas. METHODS : This study was an observational study with cross sectional comparative design. Samples faeses of infant was taken by Multistage Simple Random Sampling with total sample of 28 infants in range 0-6 months. This study was conducted in Pauh community health center areas, Village Andalas . The infant’s feces was taken then send to Microbiology Laboratorium of Dr. M. Djamil Hospital Padang city, Indonesia. The instruments used were a questionnaire and a colony counter. Data were processed and analyzed by using independent t test and Mann Whitney test. RESULTS : The fecal microbiota of the 0-6 months Indonesian infants village andalas two species including bifidobacterium and eschericia coli were common commensal intestinal microbiota in all infants. The predominant intestinal microbiota in the breast fed infants bifidobacterium average 3,59 x 109 CFU/g (p < 0,05). the mean eschericia coli microbiota from the formula fed infants 66,8 x 109 CFU/g (p < 0,05) CONCLUSION : Exclusive breastfeeding infant’s faeses contain more bifidobacteria and formula breastfed infant’s faeces contain more escherichia coli bacteria. These findings advance our understanding of the gut microbiota in healthy infants. They also provide new evidence infant diet as determinants of this essential microbial community in early life
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