The southern cowpea weevil, Callosobruchus maculatus (F) is a severe agriculture pest worldwide. In the current work, newly emerged adult males of C. maculatus have been irradiated with a low dose of 20Gy gamma radiation. The inherited deleterious effects on the fecundity, hatchability, adult emergence, and the sterility percent were recorded for the progenies F1 and F2 of the irradiated parental males. The fecundity, hatched larvae, the number of males and females were reduced in both F1 and F2. The sterility percent was high in F1 (70.8%) and increased in the F2 (88.3%) generation. Histopathological effects were also documented in the testes of F1 and F2 progenies. The spermatids and sperms have exhibited a variety of abnormalities. In the early spermatids, the nebenkern outer cell membrane was ruptured. The spermatid nucleus loses its homogeneous texture and has multiple foci of dense chromatin, as well as, profiles range has little dense material. In some groups, the nucleus had a peculiar ring of chromatin. The sperms had shown a variety of aberrations. The sperms irregularity distributed in lysed cysts by unusual manner. Also, some sperms had remarkably enlarged axoneme and small rounded nucleus. Many of the sperm cells were observed with two axonemes, abnormal mitochondria derivatives, and more than two accessory bodies. These results indicate that the low dose of 20 Gy induces semi-sterility in C. maculatus through generations. The same technique would help to improve using of sterile insect technique for other agriculture pests.

Larvicide UltrasonicationSynthetic insecticides cause pollution to the environment. In addition, insects develop resistance toward them. So, there is an urgent need for effective safe alternatives. In the current study, nanoemulsion was prepared from essential oil of Cirtus sinensis by the ultrasonic method. The efficacy of the nanoemulsion was evaluated against larvae of Culex pipiens and compared with that of the bulk emulsion. The mean droplet size of the nanoemulsion was 78.8±14.2 nm with poly dispersity index (PDI) value 0.28. The LC 50 for the nanoemulsion and the bulk emulsion were 27.4 and 86.3 ppm, respectively related to Citrus sinensis essential oil. The Larvicidal activity of the formulated nanoemulsion was more toxic than that of bulk emulsion. The results showed that nanoemulsion of Cirtus sinensis EO can be used for control of vector-borne disease Culex pipiens larvae.

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