Salt tolerance of rice is of great importance in agricultural production, since productivity of grain grown on saline lands largely depends on it. The paper deals with determining the presence of salt tolerance gene “Saltol” in rice hybrids obtained from crossing contrasting samples. To analyze salt tolerance, there were used seeds germinated in cups with distilled water and 1.5% NaCl solution, after which 10-day-old seedlings were weighed and their ratio to the control was determined. As a result of studies, there have been identified salt tolerant lines carrying the gene “Saltol” in a homozygous state. In the control nursery there were studied the best samples on productivity and elements of its structure. Two lines “7328” and “7322” were selected from the hybrid IR 52713-2B-8-2B-1-2 x Novator which significantly exceeded the standard variety “Yuzhanin” on 0.57-1.28 t/ha. On average for 2 years, they produced 6.82-7.53 t/ha (6.25 t/ha of the standard variety).
Балкарский государственный университет имени Х.М. Бербекова». В ж урнале печатаю тся ранее неопубл икованны е работы проблемного, экспериментального и методического характера по важнейшим фундаментальным и прикладным направлениям биологической, сельскохозяйственной и технической науки. С 22 марта 2018 г. журнал включен в утвержденный ВАК Перечень рецензируемых научных изданий, в которых должны быть опубликованы основные научные результаты диссертаций на соискание ученой степе ни кандидата наук, на соискание ученой степени доктора наук. Т е м а ти ч е ски е н а п р а в л е н и я ж ур н а л а :
The article presents the results of the analysis of salt tolerance in rice samples by the laboratory methods at a concentration of NaCl – 1,5 %. The plants were grown in an incubator at temperature 26 °C. After 15 days, we measured the length of roots and shoots; the germination of seeds was counted. For the analysis of hybrids sensitive to salt Krasnodar varieties Novator with lines IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, NSIC Rc 106 – donor locus salt tolerance Saltol, using markers RM 493 and RM 7075. 90 plants were selected from the F2 plants which are analyzed by PCR, and the results of DNA analysis identified 6 of them as salt tolerant samples homozygous for the desired locus. From their offspring was selected the promising form to create salt-tolerant rice sorts.
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