This study aims to determine the association between prostate volume and PSA levels in BPH patients at Ulin Hospital Banjarmasin. This study used a cross-sectional retrospective approach and analytic method. A total of 52 samples were obtained by purposive sampling based on inclusion and exclusion criterias. The highest prostate volume of 20.00-39.99 cc was obtained from 16 patients (30.7%) and the lowest volume of 60.00-79.99 cc was obtained from 10 patients (19.2%). Examination of PSA levels were highest at 2.6-9.9 ng/mL from 24 patients (46.2%) and the lowest at >20.0 ng/mL (5.8%) from 3 patients. Data analysis using Pearson correlation coefficient showed a positive correlation between prostate volume and PSA levels in BPH patients at Ulin Hospital Banjarmasin with a p value = 0,000 and r = 0.616 (positive and strong correlation). Keywords: BPH, Prostate volume, PSA
BACKGROUND: Prostate cancer is the second most common malignancy in men and has become the sixth leading cause of death in males worldwide. Eurycoma longifolia Jack root has active compounds, namely, quassinoids, eurycomanone, and canthine, which have potential as detoxicants, free radical antioxidants, and anticancer. AIM: This study aimed to analyze the potential of the active compounds in E. longifolia Jack root in induce apoptosis in the prostate adenocarcinoma PC-3 cells. METHODS: E. longifolia root active compounds were obtained by extracting them using ethanol solvent. The culture of prostate cancer PC-3 cell line was obtained from androgen-independent prostate adenocarcinoma with bone metastasis use as subject. Examination of the potency of E. longifolia root extract was conducted by observing the cells undergoing apoptosis with TUNEL assay. RESULTS: One-way ANOVA test showed that the increase in apoptotic cells was associated proportionally with the concentration levels of E. longifolia root extract and showed a significant difference (α < 0.05). CONCLUSION: The higher the dose of E. longifolia root extract, the higher will be the apoptotic level of adenocarcinoma cells PC3. E. longifolia extract is potentially used in the treatment of prostate cancer by inducing apoptotic mechanisms.
Active compound of Pasak Bumi’s root, a native plant of Indonesia, is used as antitumor by triggering cell apoptosis and reactivating the silence tumor suppressor gene caused by hypermethylation, inhibiting cancer cell proliferation. The aim of this study is to know the role of quassinoid from Pasak Bumi’s root (Eurycoma longifolia Jack) as an inhibitor of CDK2 methylation in silico. This is a descriptive study. CDK2 samples are obtained from Protein Data Bank (RSCB.org) with ID3SWR, samples of natural quassinoid are obtained from PUBCHEM NCBI and controlled by Sunitinib (®Sutent). Autodock Vina program PyRx 0.8 is used to analyze Molecular Docking. The process of analyzing molecular interactions is carried out using the LigandScout V.2.0 program. Visualization process are carried out using LigandScout V.2.0 program. The affinity of quassinoid and sutinab to CDK are -6.1 and -9.4, respectively. The more negative the binding affinity value, the better the ability of the compound (ligand) to bind to the receptor (macromolecules). From this case, Sutinab has better value compared to quassinoid. Target protein analysis using HITPICK shows quassinoid’s target predictor is JUN protein. Protein interaction analysis are obtained, and the compound is using stitch. JUN protein and Sunitib could bind with CDK2. The conclusion of this study is Sutinib has greater affinity compared to quassinoid.
Benign prostatic hyperplasia (BPH) is an enlargement of prostate gland caused by hyperplasia of prostate cells. There are several studies about BPH from histopathological aspect, but none has discussed about the relation with incidence of urinary retension which is a symptoms of worsening BPH. The purpose of this study is to analyze the relation of percentage of stroma, epithelium, and lumen to incidence of urinary retention in BPH at Ulin General Hospital Banjarmasin in 2018-2019. The studies collected 78 samples.The result showed 47 patients with urinary retention and 31 patients without urinary retention (LUTS). The mean percentages of stroma, epithelium, and lumen in the urinary retention group is 62.3 ± 10%, 18 ± 6%, and 19 ± 7% , while those in the LUTS group is 63,6 ± 13%, 14 ± 7%, and 22 ± 10%. Analysis result used unpaired t test showed no significant difference between percentage of stroma (p = 0.636), epithelium (p = 0.11), and lumen (p = 0.183) to incidence of urinary retention in BPH. This may be due to several factors in which the researcher did not pay attention to when taking samples. Keywords: benign prostatic hyperplasia, percentage of epithelium, percentage of lumen, percentage of stroma, urinary retention Abstrak: Benign prostatic hyperplasia (BPH) merupakan penyakit pembesaran kelenjar prostat yang disebabkan oleh hiperplasia sel-sel prostat. Terdapat beberapa penelitian yang meneliti BPH dari aspek histopatologi berupa stroma, epitel, dan lumen, tetapi belum ada penelitian yang mengkaitkan histopatologi BPH dengan kejadian retensi urine yang merupakan gejala bertambah parahnya BPH. Penelitian ini bertujuan untuk mengetahui hubungan persentase stroma, epitel, dan lumen dengan kejadian retensi urine pada BPH di RSUD Ulin Banjarmasin periode 2018-2019. Terdapat 78 sampel pada penelitian ini. Didapatkan sebanyak 47 pasien (60,25%) retensi urine dan 31 pasien (39,75%) non retensi urine/LUTS. Rata-rata persentase stroma, epitel, dan lumen pada kelompok retensi urine adalah 62,3 ± 10%, 18 ± 6%, dan 19 ± 7%, sedangkan dari kelompok LUTS adalah 63,6 ± 13%, 14 ± 7%, dan 22 ± 10%. Hasil analisis menggunakan uji t tidak berpasangan menunjukkan tidak terdapat hubungan antara persentase stroma (p = 0,636), epitel (P = 0,11), dan lumen (P = 0,183) terhadap kejadian retensi urin pada BPH. Hal ini kemungkinan disebabkan oleh beberapa faktor yang tidak diperhatikan peneliti saat mengambil sampel. Kata-kata kunci: benign prostatic hyperplasia, persentase epitel, persentase lumen, persentase stroma, retensi urine
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