Fibre type composition of skeletal muscle in horses varies due to factors like breed, age and training. Variations due to these factors in muscle fibre type composition of the Swedish cold-blooded trotter, a heavier and slower breed than the Standardbred trotter, have not previously been reported. The objective of this study was therefore to investigate muscle fibre type composition of young and racing cold-blooded trotters using both histochemical and immunohistochemical methods. Muscle biopsies (gluteus medius) were obtained from 2-year-old (n ¼ 18) and 4-8-year-old horses (n ¼ 6). Four of the 2-year-old horses were sampled again during their 4-yearold season. All of the horses were in professional training and the group included superior racehorses. Fibre types (I, IIA and IIB) were visualized with the myosin ATPase technique. Some samples were also stained immunohistochemically to identify MHCI, MHCIIA and MHCIIX fibres using myosin heavy chain (MHC) antibodies. The oxidative capacity of the fibres was subjectively evaluated from NADH-tetrazolium reductase stains. Type I and type IIA fibres were all identified as MHCI and MHCIIA fibres, respectively, whereas type IIB fibre population included both pure MHCIIX and hybrid MHCIIAX fibres. The older racehorses had a higher proportion of type MHCIIA and a lower proportion of type MHCIIX fibres than the 2-year-old horses. Areas of type I fibres were larger and those of type IIB fibres were smaller in racing horses compared with young horses. The proportion of type MHCIIX fibres that stained medium for oxidative capacity increased with age and training. In conclusion, training and racing induce muscular adaptations in cold-blooded trotters as in other breeds. Furthermore, immunohistochemical methods detect hybrid fibres indicating that transitions of fibre types may occur.
It is generally accepted that gastrointestinal short-chain fatty acids (SCFAs) Á/ acetic, propionic and butyric acid Á/ are mostly derived from carbohydrates, while iso-butyric and iso-valeric acids are from protein sources. We have investigated the faecal SCFAs and the correlation between the branched-chain fatty acids iso-butyric and iso-valeric in humans, rats, horses and pigs. The subjects were of different ages, fed on different diets and housed in different environments. High differences in the total output of SCFAs were observed within and between species. Despite these differences, a remarkable correlation between the iso-butyric and the iso-valeric acids was found. The fact that the correlation is strong irrespective of species, age, diet and living conditions indicates a common source actually reaching the hindgut. We hypothesize that this source is intestinal sloughed cells.
The clinical effects of intra-articular betamethasone together with hyaluronan (betaM/HA) and treatment with a defocalized carbon dioxide laser on acute traumatic arthritis of the fetlock joint were assessed. The horses in these studies were selected using a thorough lameness examination, including intra-articular anaesthesia abolishing the lameness. This investigation comprised an observer-blind study, including 10 sport horses (10 joints), and a prospective study, including 180 sport horses (333 joints). In both studies, the material was divided into two groups treated with either betaM/HA or a carbon dioxide laser. The treatment doses were 12 mg of betaM, 20 mg of HA or 60 J/cm2 of treated area. Convalescence before training was 21 days for both groups in the observer-blind study. In the prospective study, convalescence in the betaM/HA group was 21 days but was only 7 days for the laser-treated group. In the observer-blind study, three of five treated joints recovered in both cohorts. In the prospective study, the groups had significantly different recovery rates--68% of the betaM/HA-treated joints and 80% of the carbon dioxide laser-treated joints. These results indicate that the defocalized carbon dioxide laser should be an applicable mode of treatment of acute traumatic synovitis in horses. However, the biochemical functions related to carbon dioxide laser treatment require further elucidation.
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