: Strychnos ligustrina stem has been empirically used by the people of West Nusa Tenggara and Bali in the treatment of malaria, tooth ache and diarrhea, but there is no scientific data that supports it. This study aims to determine and prove the antibacterial activity of Strychnos ligustrina methanol extract to pathogenic bacteria in vitro and in vivo. This research is an experimental study conducted by measuring the inhibition zone (mm) growth of pathogenic bacteria, determining minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) in vitro, and determining the percentage of antibacterial activity of methanol extract of S. ligustrina stem in vivo. The experiment was conducted using 4 groups of concentrations of S. ligustrina stem methanol extract in an in vitro study of 25, 50, 75, and 100% with ciprofloxacin as a positive control and aquadest as a negative control. In vivo studies experiments were carried out using 6 treatment groups of test animals male mice Balb / c (Mus musculus). The in vitro test results showed that methanol extract of S. ligustrina stems was able to inhibit the growth of pathogenic bacteria with medium categories of clinical isolates of Staphylococcus aureus and categories of weaks to Klebsiella pneumonia and Escherichia coli isolates. The minimum inhibitory concentration (MIC) for S. aureus and K. pneumonia bacteria isolates was at a concentration of 25% while for E. coli isolates at a concentration of 30%. The methanol extract of the S. ligustrina stem has no killing power against the pathogenic bacteria tested. Antibacterial activity in vivo was able to inhibit the growth of S. aureus pathogenic bacteria by 6.60% (at 25% concentration), 8.62% (at 50% concentration), and 17.31% (at 100% concentration), against K. pneumonia was 11.85% (at 25% concentration), 51.21% (at 50% concentration), and 65.92% (at 100% concentration), against E. coliat 19.18% (at concentration 25%), 29.98% (at 50% concentration), and 40.88% (at 100% concentration). Methanol extract of S. ligustrina stem proved to have antibacterial activity in vitro and in vivo. Key words: Srychnos ligustrina, pathogenic bacteria, antibacterial, in vitro, in Vivo. Abstrak : Strychnos ligustrina secara empiris telah digunakan oleh masyarakat Nusa Tenggara Barat dan Bali dalam pengobatan penyakit malaria, sakit gigi, dan diare, tetapi belum ada data ilmiah yang mendukung. Penelitian ini bertujuan untuk menentukan dan membuktikan aktivitas antibakteri ekstrak metanol batang bidara laut terhadap bakteri patogen secara in vitrodan in vivo. Penelitian ini merupakan penelitian eksperimental yang dilakukan dengan mengukur zona hambat (mm) pertumbuhan bakteri patogen, menentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) secara in vitro, serta menentukan persentase aktivitas antibakteri ekstrak metanol batang bidara laut secara in vivo. Percobaan dilakukan menggunakan 4 kelompok konsentrasi ekstrak metanol batang bidara laut pada penelitian in vitro yaitu 25, 50, 75, dan 100% dengan ciprofloxacin sebagai kontrol positif serta aquadest sebagai kontrol negatif. Pada penelitian in vivo percobaan dilakukan menggunakan 6 kelompok perlakuan hewan uji mencit jantan galur Balb/c (Mus musculus). Hasil uji in vitro menunjukkan ekstrak metanol batang bidara laut mampu menghambat pertumbuhan bakteri patogen dengan kategori sedang terhadap Staphylococcus aureus isolat klinis dan kategori lemah terhadap Klebsiella pneumonia dan Escherichia coli isolat klinis. Nilai konsentrasi hambat minimum (KHM) untuk isolat bakteri S. aureus dan K. pneumoniae adalah pada konsentrasi 25% sedangkan untuk isolat E. coli pada konsentrasi 30%. Ekstrak metanol batang bidara laut tidak memiliki daya bunuh terhadap bakteri patogen yang diuji. Aktivitas antibakteri secara in vivo mampu menghambat pertumbuhan bakteri patogen S. aureus sebesar 6,60% (pada konsentrasi 25%), 8,62% (pada konsentrasi 50%), dan 17,31% (pada konsentrasi 100%), terhadap K. pneumonia sebesar 11,85% (pada konsentrasi 25%), 51,21% (pada konsentrasi 50%), dan 65,92% (pada konsentrasi 100%), terhadap E. coli sebesar 19,18% (pada konsentrasi 25%), 29,98% (pada konsentrasi 50%), dan 40,88% (pada konsentrasi 100%). Ekstrak metanol batang bidara laut terbukti memiliki aktivitas antibakteri secara in vitro dan in vivo. Kata kunci: Srychnos ligustrina, bakteri patogen, antibakteri, in vitro, in vivo
pengetahuan kepada seluruh siswa tentang penyakit kulit skabies meliputi pengertian, gejala, tanda, cara penularan, dan pengobatan serta pencegahannya. Manfaat diselenggarakannya kegiatan ini untuk meningkatkan pemahaman siswa/masyarakat pesantren tentang penyakit scabies dan mampu menerapkan pola hidup sehat serta lingkungan bersih dalam kehidupan sehari-hari. Kegiatan penyuluhan ini merupakan suatu upaya untuk menangani permasalahan penyakit kulit yang sering terjadi di lingkungan pondok pesantren MA. Ishlahil Athfal dengan target sasaran adalah guru dan siswa. Langkah-langkah pelaksanaan kegiatan adalah melakukan pertemuan dengan kepala sekolah dan perwakilan guru Pondok Pesantren Ma. Ishlahil Atfhfal Rumak Lombok Barat guna meminta izin melakukan kegiatan penyuluhan dan membicarakan masalah teknis pelaksanaan. Dari hasil diskusi didapatkan kesepakatan kegiatan penyuluhan dilaksanakan pada hari sabtu, 23 maret 2019 pukul 09.00 s/d selesai dengan target siswa kelas XII sebanyak 31 orang dengan tema Penyuluhan penanganan penyakit kulit (skabies). Kegiatan penyuluhan berjalan dengan lancar dimana para siswa sangat antusias dalam memperhatikan materi yang disampaikan, ada beberapa pertanyaan yang ternyata juga diajukan oleh para siswa karena rasa keingintauannya. Para siswa cukup terkejut dan takut saat melihat beberapa contoh penyakit kulit skabies yang ada dalam presentasi kami. Oleh karena itu penyuluhan tentang penyakit kulit skabies ini mampu memberikan tambahan pengetahuan bagi para siswa sehingga mereka dapat mencegah terinfeksi dan penularan penyakit skabies tersebut. Keywords: Penyakit Scabies; Penyuluhan; Penanganan
This study aimed to optimize the medium compositions and cultural conditions for improved chitinase production by a potential strain of Bacillus isolated from the marine environment and determine the antifungal activity of its chitinase against plant pathogenic fungi. Five potential isolates were cultured for chitinase production by submerged fermentation using colloidal chitin in a liquid medium. In this study, chitinase activity was determined by measuring reducing sugars, which were determined by the 3,5-dinitrosalicylic acid (DNS) assay. The most potential isolate, B26, showed similarity to Bacillus paramycoides based on the 16S rRNA gene sequence. The maximum chitinase production was achieved at 6.52±0.02 U/mL after 72 h of incubation in a medium containing 2% squid pen powder, supplemented with 0.5% sodium nitrate and 2% NaCl, with an initial pH of 7. It was observed that the optimization of cultural conditions resulted in 2.83 times higher chitinase production than an unoptimized medium. The antifungal activity of crude chitinase against phytopathogenic fungi was evaluated by a well-diffusion method. The chitinase of B. paramycoides B26 effectively inhibited the growth of Fusarium solani TISTR 3436 (83.4%) and Penicillium chrysogenum TISTR 3554 (80.12%).
The tuberculosis treatment control program is constrained by the outbreak of TB that is resistant to anti-tuberculosis drugs (OAT), especially multidrug-resistant tuberculosis. Confirmation of tuberculosis drug resistance really needs to be done in each area considering that there are variations in phenotypes and genotypes in each region through laboratory tests such as molecular biology tests. Aim of study to determine the prevalence of Mycobacteriumtuberculosis resistance to pyrazinamide antibiotics by PCR technique. This research is a descriptive exploratory study which has been carried out in the molecular biology laboratory of West Nusa Tenggara Provincial Public Hospital. The sample in this study was positive TB sputum obtained from Patut Patuh Patju Hospital The Province of West Nusa Tenggara. The results showed that 5 of the samples were pyrazinamide resistant. Concluded that Resistance to pyrazinamide antibiotics was found from 10 samples, 5 of which had developed resistance to pyrazinamide antibiotics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.