Potential of marine chitinolytic<i>Bacillus</i>isolates as biocontrol agents of phytopathogenic fungi

Kurniawan, Edy; Panphon, Somrak; Leelakriangsak, Montira

doi:10.1088/1755-1315/217/1/012044

Cited by 8 publications

(10 citation statements)

References 11 publications

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“…As shown in Table 1, isolate B26 showed the most potent strain with chitinase activity of 0.753 U/mL. The results were consistent with a previous study 19 that isolate B26 showed the highest chitinase activity observed by clear-zone formation on the colloidal chitin agar. Nevertheless, no significant difference in the chitinase production by the Bacillus isolates was observed.…”

Section: Screening Of Chitinase-producing Bacillus Isolatessupporting

confidence: 90%

“…The selection of five isolates used in this study was based on their chitinase and antifungal activity, as explained in the previous experiment. 19 Bacterial isolates were grown in nutrient agar (HiMedia, India) enriched with 3% NaCl and incubated at 30°C for 24 h. The nine pathogenic fungi were obtained from the Thailand Institute of Scientific and Technological Research (TISTR). The fungal strains were Alternaria alternata TISTR 3435, Aspergillus flavus TISTR 3041, A. fumigatus TISTR 3108, A. niger TISTR 3130, Cladosporium cladosporioides TISTR 3285, Fusarium solani TISTR 3436, Mucor ruber TISTR 3006, Penicillium chrysogenum TISTR 3554, and Rhizopus stolonifer TISTR 3016.…”

Section: Preparation Of Microorganismsmentioning

confidence: 99%

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Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Kurniawan¹,

Leelakriangsak²,

Panphon³

2022

J. Pure Appl. Microbiol.

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This study aimed to optimize the medium compositions and cultural conditions for improved chitinase production by a potential strain of Bacillus isolated from the marine environment and determine the antifungal activity of its chitinase against plant pathogenic fungi. Five potential isolates were cultured for chitinase production by submerged fermentation using colloidal chitin in a liquid medium. In this study, chitinase activity was determined by measuring reducing sugars, which were determined by the 3,5-dinitrosalicylic acid (DNS) assay. The most potential isolate, B26, showed similarity to Bacillus paramycoides based on the 16S rRNA gene sequence. The maximum chitinase production was achieved at 6.52±0.02 U/mL after 72 h of incubation in a medium containing 2% squid pen powder, supplemented with 0.5% sodium nitrate and 2% NaCl, with an initial pH of 7. It was observed that the optimization of cultural conditions resulted in 2.83 times higher chitinase production than an unoptimized medium. The antifungal activity of crude chitinase against phytopathogenic fungi was evaluated by a well-diffusion method. The chitinase of B. paramycoides B26 effectively inhibited the growth of Fusarium solani TISTR 3436 (83.4%) and Penicillium chrysogenum TISTR 3554 (80.12%).

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Section: Screening Of Chitinase-producing Bacillus Isolatessupporting

confidence: 90%

Section: Preparation Of Microorganismsmentioning

confidence: 99%

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Kurniawan¹,

Leelakriangsak²,

Panphon³

2022

J. Pure Appl. Microbiol.

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“…Trichoderma would establish a parasitic relationship with a pathogenic fungus after successful recognition, and show substantial parasitic ability in the penetration of pathogenic fungal mycelia, and the production of cell wall degrading enzymes including beta-1,6-glucanase and protease, could finally lead to death of pathogenic fungus [31,32]. In addition, microbial antagonists, such as Trichoderma harzianum and T. asperellum, are widely applied because of their ability to control plant fungal diseases that were induced by Fusarium solani, Rhizoctonia solani and Sclerotinia sclerotiorum [14,29,33]. In the present study, the B. adusta M1 hyphae had directly penetrated the FOC hyphae, which might cause the latter to swell, deform and even rupture, showing a strong mycoparasitism (Fig.…”

Section: Discussionmentioning

confidence: 99%

Bjerkandera adusta M1 inhibits Fusarium oxysporum and prevents the wilt incidence in Brassica napus

Feng

Li²,

et al. 2019

Preprint

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Background: Bjerkandera adusta degrades polycyclic aromatic compounds, such as cellulose and lignin, with the production of laccase and peroxidase. However, its effect on plant disease is unknown. Results: In this study, both the confrontation culture and greenhouse pot experiments were carried out to address the biocontrol mechanisms of B. adusta M1, which was isolated from a unique purple soil (Eutric Regosol), on the growth of pathogenic Fusarium oxysporum f. sp. conglutinans (FOC) and incidence of fusarium wilt in Brassica napus. Results showed that the hyphal growth rate of B. adusta M1 was significantly greater than that of FOC, indicating a strong competitiveness by B. adusta M1. In addition, the B. adusta M1 fermentation broth significantly inhibited the growth of FOC hyphae by 62.79±1.80%, which was greater than an inhibition rate of 40.63±1.68% by the chemical fungicide carbendazim. The image from a scanning electron microscope showed the hyphae of FOC directly was penetrated by the B. adusta M1 hyphae, indicating a strong mycoparasitism by B. adusta M1. Besides, both the B. adusta M1 and B. adusta M1 fermentation broth reduced the incidence and disease index of the fusarium wilt in Brassica napus leaves, and the control effects of different treatments against fusarium wilt were 57.09% and 47.67%, respectively, which were comparable better to 46.11% of the chemical fungicide carbendazim. Furthermore, both the B. adusta M1 and B. adusta M1 fermentation broth increased the activity of superoxide dismutase, catalase, peroxidase and phenylalanine ammonia-lyase, which related to an enhancement of disease resistance. Similarly, both the B. adusta M1 and B. adusta M1 fermentation broth decreased the cell membrane permeability and malondialdehyde contents, thereby reducing the cell membrane damage from the pathogenic fungus. Conclusion: In summary, results from this present study demonstrated that both the Bjerkandera adusta M1 and B. adusta M1 fermentation broth inhibited the growth of FOC and decreased the incidence and disease index of the fusarium wilt disease in Brassica napus. Therefore, B. adusta M1 could be applied as a potential biocontrol fungus to against the fusarium wilt disease.

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“…The confrontation culture method was used for antifungal activity determination (Kurniawan et al, 2018 ). An agar block (5 mm in diameter) from the margin of 5-day-old culture of the fungal pathogen was placed in the center of the PDA medium.…”

Section: Methodsmentioning

confidence: 99%

Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt

et al. 2022

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Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases. Microbial keystone taxa were proposed as promising targets in plant disease control. In this study, we obtained an antagonistic Bacillus isolate EM-1 from bacterial wilt-suppressive soil, and it was considered rhizosphere-resident bacteria based on high (100%) 16S rRNA gene similarity to sequences derived from high-throughput amplicon sequencing. According to 16S rRNA gene sequencing and MLSA, strain EM-1 was identified as Bacillus velezensis. This strain could inhibit the growth of R. solanacearum, reduce the colonization of R. solanacearum in tobacco roots, and decrease the incidence of bacterial wilt disease. In addition, strain EM-1 also showed a strong inhibitory effect on other phytopathogens, such as Alternaria alternata and Phytophthora nicotianae, indicating a wide antagonistic spectrum. The antimicrobial ability of EM-1 can be attributed to its volatile, lipopeptide and polyketide metabolites. Iturin A (C14, C15, and C16) was the main lipopeptide, and macrolactin A and macrolactin W were the main polyketides in the fermentation broth of EM-1, while heptanone and its derivatives were dominant among the volatile organic compounds. Among them, heptanones and macrolactins, but not iturins, might be the main potential antibacterial substances. Complete genome sequencing was performed, and the biosynthetic gene clusters responsible for iturin A and macrolactin were identified. Moreover, strain EM-1 can also induce plant resistance by increasing the activity of CAT and PPO in tobacco. These results indicated that EM-1 can serve as a biocontrol Bacillus strain for tobacco bacterial wilt control. This study provides a better insight into the strategy of exploring biocontrol agent based on rhizosphere microbiome.

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Potential of marine chitinolyticBacillusisolates as biocontrol agents of phytopathogenic fungi

Cited by 8 publications

References 11 publications

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Antifungal Chitinase Production by Bacillus paramycoides B26 using Squid Pen Powder as a Carbon Source

Bjerkandera adusta M1 inhibits Fusarium oxysporum and prevents the wilt incidence in Brassica napus

Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt

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