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Context: Based on its antioxidant activity, melatonin was recently found to have a protection effect against photocarcinogenesis. Objective: This work aimed to develop an innovative sunscreen formulation based on the Pickering emulsions concept, stabilized by physical UV filters, modified starch and natural oils associated to melatonin as a key strategy for prevention against UV-induced skin damage. Materials and methods: For this purpose, melatonin was incorporated in Pickering emulsions that were characterized using physicochemical, in vitro and in vivo testing. Physicochemical studies included physical and chemical stability by a thorough pharmaceutical control. The possible protective effects of melatonin against UV-induced cell damage in HaCaT cell lines were investigated in vitro. The safety assessment and the in vivo biological properties of the final formulations, including Human Repeat Insult Patch Test and sunscreen water resistance tests were also evaluated. Results and discussion: These studies demonstrated that melatonin sunscreen Pickering emulsion was beneficial and presented a powerful protection against UVB-induced damage in HaCat cells, including inhibition of apoptosis. The inclusion of zinc oxide, titanium dioxide, green coffee oil and starch ensured a high SPF (50+) against UVA and UVB. Conclusion: The combination of melatonin, multifunctional solid particles and green coffee oil, contributed to achieve a stable, effective and innovative sunscreen with a meaningful synergistic protection against oxidative stress.

Green coffee oil and modified starch were recently found to have an enhanced protection effect against UV radiation. Therefore, this work aimed to develop an innovative sunscreen formulation based on Pickering emulsions concept, i.e., surfactant-free emulsions stabilized by physical UV filters associated natural oils as a key strategy for prevention against UV-induced skin damage. The Pickering emulsions of different compositions were characterized in terms of pH, mechanical, physical and microbiological stability by a thorough pharmaceutical control. In addition, the sun protection factor (SPF) as well as the in vitro and in vivo biological properties of the final formulations, including Episkin®, HRIPT and sunscreen water resistance. Formulation studies demonstrated the addition of physical UV filters was beneficial, leading to the inclusion of ZnO and TiO2 to ensure a high SPF against UVA and UVB, respectively. Although starch particles presented no intrinsic photoprotection properties, they proved to be a SPF promoter by a synergistic effect. Green coffee oil was the selected natural oil due to the highest SPF, when compared to other natural oils tested. Besides the excellent sunscreen activity confirmed by in vitro and in vivo results, the final formulations proved to be also suitable for topical use according to the rheological assessment and stability throughout the study period (3months). In conclusion, the combination of three multifunctional solid particles and green coffee oil, contributed to achieve a stable and effective innovative sunscreen with a wide range of UV radiation protection.

Non-steroid anti-inflammatory drugs (NSAIDs), such as etofenamate, are among the most prescribed drugs used for their analgesic, anti-rheumatic, antipyretic and anti-inflammatory properties. Topical formulations have the main advantage of targeted delivery. However, drugs must overcome the skin due to its role as a physical and chemical barrier against the penetration of chemicals and microorganisms. This barrier must be altered to allow the permeation of drugs at a suitable rate to the desired site of activity. Permeation modulators can intercalate the skin outer layers causing structure disruption, opening an energetically favourable route for the drug to diffuse through. The aim of this work was the development of hydroalcoholic gels containing 5.0% (w/w) of etofenamate for topical administration with anti-inflammatory activity and enhanced drug delivery. The physical and chemical characterization, in vitro release and permeation studies and in vivo anti-inflammatory activity were assessed. The gel with 30% ethanol showed in vivo anti-inflammatory activity with suitable physical chemical and microbiologic characteristics. In vitro release and permeation studies revealed that the different amounts of ethanol used influenced the release profiles of etofenamate. Moreover, it was demonstrated that this formulation is an adequate vehicle for the etofenamate skin permeation.

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Conflicts stemming from the dispute over natural resources have become increasingly more frequent. Extreme weather events have intensified and have equally become more frequent owing to global warming. Although the consequences of such phenomena have repercussions on public safety, the processes of analysis incorporating the environmental dimension in this context have not been fully understood. This paper assessed how systems of environmental protection interconnect with those of crime prevention, forming the environmental safety system. It is an exploratory-descriptive research carried out through questionnaires evaluated by State environmental control agencies, exploring eight major domains: their origins; their staff, distribution and organization; logistics and operational structures; the use of geotechnologies; integration; operational results; regional analysis; and the general analysis of military environmental police. The last section, in conclusion, reviews the main lessons learned and proposes future lines of research that can be developed based on this topic. It highlights that the military environmental police should be the most significant agent of nature protection in the country. It reveals the existence of weaknesses and opportunities for improvement to be implemented.

Por meio de uma avaliação da efetividade das ações da polícia militar ambiental e dos principais postulados jurídicos associados à proteção ao ambiente, buscou-se com o presente trabalho propor uma metodologia para diagnosticar os principais óbices da não aplicação de sanções penais, administrativas ou ainda de reparação do dano, por meio de métodos descritivos e exploratórios de fundamentação empírica-fenomenológica e dados secundários quantitativos coletados nos arquivos do Comando de Polícia Ambiental da Polícia Militar do Rio de Janeiro e do Tribunal de Justiça do Estado. Constatou-se a prevalência da impunidade dos infratores e a aceleração da degradação ambiental.

allows preparing w/o emulsions with the obvious benefits of avoiding the drawbacks often associated with antimicrobial preservatives and surfactants agents.