Microbial fuel cells make it possible to generate electricity by eliminating and using wastewater components. For these purposes, the most viable microbial associations in microbial fuel cells. The presence of strains of different microorganisms allows you to consume a much wider range of compounds. Multicomponent makes them more resistant to the negative effects of stressful environmental factors. An example of such associations are silt sediments of biological treatment plants. The purpose of this work was to create a new microbial association. Microbial association was formed from previously isolated strains from activated sludge from the petrochemical plant, Micrococcus luteus and Serratia marcescens. Model wastewater was taken as substrates. The generation of electricity was carried out in microbial fuel cell models that we developed. These designs allowed access to the contents of microbial fuel cells without violating sterility and anaerobic conditions in the anode chamber. In the experiments, the voltage, current in microbial fuel cells were measured and the power was calculated. Experiments have shown high electrogenic activity when working together with Micrococcus luteus and Serratia marcescens cohosts for a good variety of the proposed bioagent complex. The search and isolation of energy-efficient strains and the compilation of a new consortium from them will not only increase the current output in microbial fuel cells to more significant values, but also bring such microbial fuel cells into the category of promising developments for alternative energy. This work was supported by the Ministry of Education and Science of the Russian Federation (project RFMEFI58317X0060 “Bioremediation and bioconversion of waste using a complex of photosynthetic organisms and heterotrophs in aerobic and anaerobic conditions with bioenergy generation”).

The purpose is: To assess the clinical relevance of the endothelial condition and platelet aggregation in the development of hepatic fibrosis in children with autoimmune hepatitis. Materials and Methods. 35 patients aged from 3 to 17 years old were studied, including 19 girls - (54%) and 16 boys - (46%). The 1st group consisted of 17 children with the degree of fibrosis F 0-2 acc. to the METAVIR score; the 2nd group consisted of 18 patients with the degree of fibrosis F 3-4 (METAVIR) acc. to the METAVIR score based on the indirect elastometry data in children of the 2nd group hepatic cirrhosis was diagnosed; the control group consisted of 15 children with health group I or II. The endothelium state and the platelet aggregation activity were assessed in all the patients. To test statistical hypotheses, the Mann-Whitney U test, the Spearman correlation coefficient and the Fisher criterion were used. The critical value of the significance level is assumed to be 0.05. Quantitative data are presented as: median and the first; third quartile of Me (Q1; Q3). Results. In children with autoimmune hepatitis some signs of the various-degree fibrosis formation were revealed in the ¾ of the examined. Patients of the 2nd group have a more aggressive course as compared to the 1st group: in the disease debut there were mainly expressed asthenoneurotic complaints (p = 0.021), manifestations of the jaundice syndrome (p = 0.014), more frequently hepatic-cell insufficiency (p = 0.045) is diagnosed and followed by complications of the disease (hypersplenism (p = 0.014), varicose veins of the esophagus (p = 0.003)). All children with autoimmune hepatitis have the endothelial dysfunction, the enhancing platelet aggregation activity. The degree of fibrosis correlates with the concentration of endothelin-1 (r = 0.4, p = 0.004), the von Willebrand factor (vWF) activity (r = 0.5, p <0.001), the platelet count (r = -0.5, p = 0.003). The determination of the endothelin-1 concentration, the von Willebrand factor activity and the platelet count may be used to assess the hepatopathy severity in children with autoimmune hepatitis. Conclusion. In children with autoimmune hepatitis the endothelial dysfunction and platelet disorders are revealed in the hemostasis system correlating with the severity of the pathological process.

The ability of a variety of commercial microbiological preparations for local wastewater purification and recycling of various solid waste to generate electricity in a microbial fuel cell for disposal of various products was investigated. The dynamics of transformation of substrates, and various changes in the environment settings anolyte and catholyte in the process of the microbial fuel cell was studied.

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One of the most promising areas in the fight against oil pollution is the use of microorganisms. When eliminating oil pollution, an important task is the selection of surfactants that facilitate the interaction of oil-oxidizing microorganisms with oil products. Dispersion of hydrophobic substances, in particular petroleum hydrocarbons, dramatically increases their surface. In turn, this significantly increases the area of interaction of hydrophobic compounds with oxygen and oil-oxidizing microorganisms. In this work, the effect of humic substances on the aggregate state of various hydrophobic substances is studied. The bioagents were taken strains: Yarrowia lipolytica and Bacillus thuringiensis. Sodium dodecyl sulfate, melanin and humates were chosen as classic surfactants. As sorbing surfaces, slides that were pretreated with silicone or paraffin were used in the work. Experiments have shown that in the presence of sodium dodecyl sulfate, the sorption of cells and spores of microorganisms significantly decreased. With longer exposures, the concentration of cells and spores on the glasses not only did not increase, but even decreased. Moreover, the higher the content of sodium dodecyl sulfate, the weaker the cells and spores of the microorganisms were bound to the hydrophobic surface, and the rate of desorption was higher. It was shown that humates at certain concentrations, like twin, dispersed diesel fuel and fuel oil, and also reduced the amount of cell adhesion. This work was financially supported by the RFBR grant 19-29-05213 mk «Mechanisms for the integrated interaction of soils with oil, oil products and surface-active substances in oil pollution and bioremediation processes».

The electrogenic activity of many microorganisms used in MFC is due to direct electron transfer. It is achieved by direct contact of microbial cells with an electrode. In this regard, an important aspect of research in the field of MFC is the study of the interaction of electrogenic microorganisms with electrode materials. In this work, the influence of polarization of carbon fiber from an external source of electrical energy on the intensity of sorption on it of cells of the electrogenic strain Micrococcus luteus 1-i is considered. It was shown that the polarization of carbon fiber 0.6-0.8 V positively influenced the sorption of cells of the electrogenic strain M. luteus 1-i, and 0.4-0.6 V positively influenced the increase in the number of cells of the strain. The preliminary application of a voltage of 0.6 V to the MFC anode for 2 h led to an increase in the current generated by the M. luteus strain 1 within a 24hour experiment. The obtained positive effect demonstrates the possibility of using this approach to increase the efficiency of the MFC technology.