Micrococcus luteus and Serratia marcescens, as a new association of bio-agents for microbial fuel cells

Abstract

Microbial fuel cells make it possible to generate electricity by eliminating and using wastewater components. For these purposes, the most viable microbial associations in microbial fuel cells. The presence of strains of different microorganisms allows you to consume a much wider range of compounds. Multicomponent makes them more resistant to the negative effects of stressful environmental factors. An example of such associations are silt sediments of biological treatment plants. The purpose of this work was to create a new microbial association. Microbial association was formed from previously isolated strains from activated sludge from the petrochemical plant, Micrococcus luteus and Serratia marcescens. Model wastewater was taken as substrates. The generation of electricity was carried out in microbial fuel cell models that we developed. These designs allowed access to the contents of microbial fuel cells without violating sterility and anaerobic conditions in the anode chamber. In the experiments, the voltage, current in microbial fuel cells were measured and the power was calculated. Experiments have shown high electrogenic activity when working together with Micrococcus luteus and Serratia marcescens cohosts for a good variety of the proposed bioagent complex. The search and isolation of energy-efficient strains and the compilation of a new consortium from them will not only increase the current output in microbial fuel cells to more significant values, but also bring such microbial fuel cells into the category of promising developments for alternative energy. This work was supported by the Ministry of Education and Science of the Russian Federation (project RFMEFI58317X0060 “Bioremediation and bioconversion of waste using a complex of photosynthetic organisms and heterotrophs in aerobic and anaerobic conditions with bioenergy generation”).

Co‐substrate‐fed microbial fuel cell for enhanced power generation and removal of groundwater contaminants

“…Previous studies on the same inoculum reported that the voltage generated with B. subtilis was only 150 mV under the 1 kΩ external load using glucose as substrate 26 . Furthermore, the voltage generation by S. marcescens from activated sludge as inoculum was only 9 mV in MFC 27 …”

“…26 Furthermore, the voltage generation by S. marcescens from activated sludge as inoculum was only 9 mV in MFC. 27 Interestingly, BTEP concentrations in all MFCs decreased to below 70 mg/L and removal efficiency approached to 97.8%, 99.5%, 99.9%, and 93.7% for BTEP, respectively, with co-substrate-fed MFCs, indicating that the most BTEP was consumed by diverse non-ARB that cannot produce electricity. The respective BTEP removal efficiency of BTEP-fed MFC was 97.6%, 99.3%, 98.8%, and 95.4%, but lower voltage was generated.…”

Changes in the number and intensity of sorption of Micrococcus luteus on carbon fiber at different degrees of its polarization

“…The highest efficiency of substrate removal (48%) was also obtained when using MFC with an applied voltage of 0.5V. This was followed by 0.3V (37%), 0.1V (32%) and control (27%) [8][9].…”

Effects of the anionactive surfactant sodium dodecyl sulfate on the adhesion of cells of the strain <i>Micrococcus luteus</i> 1-i to the surface of carbon materials

“…Ранее он был выделен нами из активного ила очистных сооружений нефтеперерабатывающего предприятия. Штамм продемонстрировал довольно высокую электрогенную активность в МТЭ [24,25]. Он депонирован во Всероссийскую коллекцию микроорганизмов (ВКМ) под номером ВКМ Ac-2637 D.…”