BIOTENSOATIVE PRODUCTION USING Pseudomonas aeruginosa (P.A.) AND AGROINDUSTRY WASTE (CASSAVA WASTEWATER) AS SUBSTRACT ABSTRACTBiosurfactants are amphoteric molecules that act preferentially on the interface of fluids of different polarities such as water and oil, and they may be synthesized by micro-organisms. Using a factorial design 2 4-1 (half fraction) 10 assays were performed using Pseudomonas aeruginosa (PA) AP029/GVII-A as microorganism in rotary incubator (shaker), in which we analyzed the influence of factors (temperature, agitation, rate of aeration and concentration of culture medium) at two different levels for the synthesis of biosurfactant. Samples were collected throughout the cultivation until we completed 132h of fermentation. We tried the best result following the production through consumption of substrate, dry matter, reduction of surface tension (ring method) and emulsification index. The results showed that the cassava wastewater is a very assimilable substrate for the production of biosurfactant, reaching 91% of consumption by the micro-organism under study. The cultivation temperature was found to be one of the leading factors in the synthesis of the metabolite, followed by aeration rate and also due to the agitation.The best results showed a reduction of 30% in surface tension (% RTS) for the medium, reaching values of 30 mN/m, 3.0 g / L of biomass and emulsification index greater than 65%.
Glucose oxidase (GOx) has several industrial applications. It is believed that there are several species of fungi that have the ability to produce this enzyme, most of which are unexplored. This work aimed to investigate the production of glucose oxidase (EC 1.1.3.4) by fungi isolated from soil samples of the Amazonian forest. Filamentous fungi were isolated from soil samples from the Adolpho Ducke Forest Reserve, located in Manaus, Amazonas. Strains were subjected to submerged bioprocessing to select for the best GOx producers. Those selected for the production of isolated enzymes were subjected to kinetic tests that evaluated production of the enzyme and consumption of the biomass substrate by the isolates. In addition, experiments to evaluate the optimal carbon, nitrogen and phosphorus sources as well as the influence of the bioprocess factors were carried out. Finally, GOx production was investigated in a semi-continuous system for 7 days. The most frequent isolates isolated from soil samples belonged to the genera Aspergillus, Penicillium and Trichoderma. Aspergillus niger LMM01 was the best GOx producer. Glucose, peptone and KH 2 PO 4 were demonstrated to be the optimal carbon, nitrogen and phosphorus sources, respectively. Multivariate experiments demonstrated that the parameters with the greatest effect on GOx production were pH and agitation. Stable expression results for GOx (7.74 U/ml) were obtained over 7 days in a semi-continuous process. In this context, the new Amazonian source of this enzyme (A. niger LMM01), and enzyme production in a semi-continuous process, demonstrates the importance of the present work.
The purpose of this study was to assess pectinase production through solidstate fermentation by Aspergillus niger IOC 4003 using caja depulping residue. Enzyme production was carried out in 250 mL Erlenmeyer flasks containing 5 g of washed caja residue or unwashed caja residue supplemented with KH2PO4 (1.5 g/L) and (NH4)2SO4 (3.3 g/L). Initial pH and moisture were adjusted for 4.5 and 40%, respectively. SSF was started after inoculation of 1 x 10 7 spores/g residue of A. niger and incubated at 30°C. The microorganism was assessed during 240 hours of cultivation where at every 24 hours the complex enzymatic were extracted with acetate buffer (pH = 4.5, 200 mM) at ratio 1:10 (g residue:mL buffer). There were measured polygalacturonase, pectin lyase and total cellulase activity as well as quantification of total protein and total reducing sugars. The best residue was WCR where it was obtained the maximum enzymatic production of PFase, PGase and PNLase activity of 3.49 ± 0.08 IU/g (48 h), 38.2 ± 0.63 IU/g (72 h), 8.58 ± 0.78 IU/g (48 h), respectively. Initial encountered TRS was 66.0 ± 0.2 mg/g for WCR. Therefore, washed caja residue demonstrated a great potential for the production of pectinolytic enzymes and using these enzymes in industrial applications.
Pectinases são enzimas que hidrolisam pectina, um polissacarídeo encontrado na parede celular de plantas. Essas enzimas são bastante utilizadas em diversos processos industriais, como por exemplo na indústria de sucos e vinhos, alimentos, papel e tecidos. O trabalho objetivou produzir pectinases secretadas pelo fungo Penicillium chrysogenum quando crescido no pedúnculo de caju como única fonte de carbono por 5 dias a 30 °C. Os resultados mostraram que a melhor condição para produção da enzima foi usando 70% de umidade, com (NH4)2SO4 (0,3%) e KH2PO4 (0,1%) com atividade de 0,39 UI/g.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.