No abstract

A simple culture technique was used to follow sheep reticulocyte maturation. Cells remained in good condition without lysis for at least 7 days and maintained normal intracellular ATP concentrations. Amino acid and nucleoside transport were measured during maturation together with changes in reduced glutathione (GSH), amino acid, K+ and Na+ concentrations. Reticulocytes of the genetic types high-K+ and low-K+, high-GSH and low-GSH; nucleoside-permeable and nucleoside-impermeable were indistinguishable both in transport properties and cellular constituents. Cell maturation was associated with marked decreases in L-alanine transport (Na+-dependent and independent), uridine transport and GSH and K+ concentrations. Typical mature-cell levels for all parameters were reached within the culture period, during which time predicted differences between cells of different genotypes became apparent. Loss of amino acid-transport activity from low-GSH cells was accompanied by an increase in intracellular ornithine and lysine concentrations. The rates at which different parameters changed during culture were compared.

The set of proposed guidelines for gene nomenclature in sheep (Ovis aries L) and goats (Ca P ra hircus L) which had been drawn up during the 1987 COGNOSAG Workshop at Gontard/Manosque, France, was revised by committees during the 1988 and 1989 COGNOSAG Workshops at Gontard/Manosque, France, and Eugene, Oregon, USA. This set has been revised in July 1991, at a workshop at Gontard/Manosque, France, to accommodate all ruminants and to facilitate the development of a comparative genome nomenclature. cattle / goat / sheep / gene nomenclature Résumé-Nomenclature des gènes des ruminants 1991. Les directives pour la nomenclature des gènes du mouton (Ovis aries L) et de la chèvre (Capra hircus L) élaborées au cours de l'Atelier du COGOVICA tenu à Gontard/Manosque en juillet 1987 ont été révisées lors des Ateliers du COGOVICA de juillet 1988 (Manosque) et de juin 1989 (Eugene, Orégon, Etats-Unis). Ces directives ont été revues en juillet 1991 au cours d'un Atelier du COGOVICA organisé à GontardlManosque en juillet 1991, de manière à être applicables à tous les ruminants et afin de faciliter les progrès de la nomenclature comparée de leur génome. bovin / chèvre / ovin / nomenclature génétique

Three NADH diaphorase phenotypes were observed in the red cells of sheep. Breeding data indicated that this polymorphism was under the control of two autosomal codominant alleles, designated DiaF and Dias. Phenotype Dia F had significantly lower NADH diaphorase activity than phenotype Dia S . The frzquency of DiaF and DiaS was determined in 9 different breeds.

SummaryThe purine nucleoside phosphorylase (NP) activity of sheep red cells was determined by starch gel electrophoresis and by a spectrophotometric assay technique. Some sheep had high activity (NP‐high type) and some had low or zero activity (NP‐low type). The enzyme deficiency is apparently confined to the red cell since other tissues from NP‐low type animals had activities similar to those from NP‐high type individuals. Family data indicated that NP activity is controlled by a pair of autosomal allelic genes, designated NPH and NPL. Sheep heterozygous for the NP genes had lower enzymic activities than homozygous high‐type individuals. The frequency of NP types in different breeds of sheep was determined. Barbary and Mouflon sheep had activities similar to NP‐high type domestic sheep; goats had high enzyme activities but their NP had a slower electrophoretic mobility than that of sheep.