NADH diaphorase as a genetic marker for sheep red cells

Abstract

Three NADH diaphorase phenotypes were observed in the red cells of sheep. Breeding data indicated that this polymorphism was under the control of two autosomal codominant alleles, designated DiaF and Dias. Phenotype Dia F had significantly lower NADH diaphorase activity than phenotype Dia S . The frzquency of DiaF and DiaS was determined in 9 different breeds.

Comparative aspects of biochemical polymorphism in the blood of Caprinae species and their hybrids

“…Separation of blood proteins was carried out by starch gel electrophoresis with previously described methods: haemoglobin (Gahne et al, 1960); red cell carbonic anhydrase and 'X' protein (Tucker et al, 1967); red cell nucleoside phosphorylase (Tucker & Young, 1976); red cell lactatee dehydrogenase , red cell NADH diaphorase 1 & 2 (Tucker & Crowley, 1978); red cell malic enzyme ; plasma albumin (Tucker, 1968); plasma transferrin (Rasmusen & Tucker, 1973).…”

“…Adult animals. Three phenotypes of isozymic NADH diaphorase 1 have been described in sheep, Dia F, Dia S and Dia FS for the electrophoretically fast, slow and heterozygous forms respectively (Tucker & Crowley, 1978;Cepica & Stratil, 1978). The diaphorase phenotypes are shown diagrammatically in Fig.…”