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Plasma from pig, sheep, goat, cattie, horse, rabbit, dog, rat, guinea pig, and chicken was examined for the presence of specific binders for folic acid. It was found that only pig plasma contains unsaturated avid specific binders of folic acid. The maximum binding capacity of pig plasma for folic acid was found to be 14.0–26.5 ng/ml of plasma. These binders have a lower affinity for other folates and folate analogues. They are partly saturated by endogenous folates and they move electrophoretically in veronal buffer, pH 8.6, in an area including β-globulin and most of the γ-globulin fraction. Specific binders of folic acid with high binding capacity were also found in one female sheep out of the 16 tested.

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SummaryDouble tracer methods using Fe59 and Cr51 for simultaneous clinical investigations of erythropoiesis and red cell destruction are described.In these methods, the clearance of intravenously injected Fe59 from the plasma and its incorporation into the red cells is followed by the assay of blood samples, whilst the turnover of Fe59 by the tissues is followed by body surface counting. At the same time, the survival of Cr51-labelled red cells in the circulating is followed by the assay of blood samples whilst sites of destruction of labelled cells are identified by body surface counting. Pulse height analysis techniques are used for simultaneous measurements of Fe59 and Cr51 both in vitro and in vivo.The use of these techniques is illustrated by results obtained in studies on normal subjects and on patients with various congenital haemolytic anaemias.

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