Glycosphingolipids of lymphoid tissues, bone marrow cells, mixed blood leucocytes, separated lymphocytes, and granulocytes from large white pigs were analyzed by thin layer and gas liquid chromatographies. The composition of the glycosphingolipids of thymus and blood leucocytes (mixed population) was similar, and trihexosyl ceramide (galactosyl‐[1–4]‐galactosyl‐[1–4]‐glucosyl‐[1–1]‐ceramide) was the major glycosphingolipid component of both tissues. The fatty acid fractions of all glycolipids from the two tissues were analyzed, and gross differences are discussed. Blood lymphocytes had a higher content of glycosphingolipids/mole phospholipid or mg protein than thymus lymphocytes obtained by a gentle washing of sliced tissue. Similar and more pronounced differences were obtained when the glycosphingolipid content of bone marrow cells (>50% polymorphonuclear neutrophils) was compared with that of blood polymorphonuclear neutrophils. In general, most of the blood leucocytes were richer in glycosphingolipids than most of the cells of the lymphoid tissues and bone marrow. These results indicate a marked difference in lipid composition between blood polymorphonuclear neutrophils and bone marrow cells. It is possible that certain of the biophysical properties which characterize blood polymorphonuclear neutrophils and which derive from changes in the cell periphery of immature granulocytes are connected with these differences in membrane lipid composition.
Although iron-binding capacity increases during pregnancy, there is no correlation of it with mean values of serum iron and haemoglobin levels. The administration of drugs (vitamins or vitamins + iron) do not influence either mean haemoglobin, serum iron levels or iron binding capacity
In a total of 55 samples of cerebrospinal fluid (CSF) and an equal number of serum samples obtained from 45 patients with neurological disorders and 10 controls, folic acid and vitamin B12 were measured. A radioisotopic assay method was used. A significant decrease of CSF folic acid was noted in the group with cerebral tumors.
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