Objective:To identify the antihyperglycemic activity of various extracts, petroleum ether (60-80°), chloroform, acetone, ethanol, aqueous and crude aqueous, of the flower buds of Michelia champaca, and to identify the antidiabetic activity of active antihyperglycemic extract.Materials and Methods:Plant extracts were tested for antihyperglycemic activity in glucose overloaded hyperglycemic rats. The effective antihyperglycemic extract was tested for its hypoglycemic activity at two-dose levels, 200 and 400 mg/kg respectively. To confirm its utility in the higher model, the effective extract of M. champaca was subjected to antidiabetic study in alloxan induced diabetic model at two dose levels, 200 and 400 mg/kg respectively. The biochemical parameters, glucose, urea, creatinine, serum cholesterol, serum triglyceride, high density lipoprotein, low density lipoprotein, hemoglobin and glycosylated hemoglobin were also assessed in the experimental animals.Results:The ethanolic extract of M. champaca exhibited significant antihyperglycemic activity but did not produce hypoglycemia in fasted normal rats. Apart from this extract, the crude aqueous and petroleum ether extracts were found active only at the end of the first hour. Treatment of diabetic rats with ethanolic extract of this plant restored the elevated biochemical parameters significantly (P<0.05) (P<0.01) and the activity was found dose dependent.Conclusion:This study supports the traditional claim and the ethanolic extract of this plant could be added in traditional preparations for the ailment of various diabetes-associated complications.

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Various extracts including petroleum ether, chloroform, acetone, ethanol, aqueous and crude aqueous of barks of Acacia catechu (A. catechu) Willd (Leguminosae) and the two fractions of ethanolic extract were tested for antihyperglycaemic activity in glucose-loaded hyperglycaemic rats. The effective extract and fraction of A. catechu were subjected to antidiabetic study in alloxan-induced diabetic rats at two dose levels, 200 and 400 mg/kg, respectively. Biochemical parameters, including glucose, urea, creatinine, serum cholesterol, serum triglyceride, high density lipoprotein (HDL), low density lipoprotein (LDL), haemoglobin and glycosylated haemoglobin were also assessed. The ethanolic extract of A. catechu and the water insoluble fraction of ethanolic extract exhibited significant anti-hyperglycaemic activity and produced dosedependent hypoglycemia in fasted normal rats. Treatment of diabetic rats with ethanolic extract and water-insoluble fraction of this plant restored the elevated biochemical parameters significantly (p<0.05) to the normal level. Comparatively, the water insoluble fraction of ethanolic extract was more effective than the ethanolic extract and the activity was comparable to that of the standard, glibenclamide (5 mg/kg).

Various extracts of flowers of Cassia fistula Linn (Leguminosae) such as petroleum ether (60-80°), chloroform, acetone, ethanol, aqueous, and crude aqueous extracts and two fractions of ethanol extract were tested for antihyperglycemic activity in glucose-overloaded hyperglycemic rats. The effective antihyperglycemic extracts and fraction were tested for their hypoglycemic activity at two dose levels, 200 and 400 mg/kg, respectively. To confirm their utility in higher models, the effective extracts and fraction of C. fistula were subjected to antidiabetic study in an alloxan-induced diabetic model at two dose levels, 200 and 400 mg/kg, respectively. Biochemical parameters like glucose, urea, creatinine, serum cholesterol, serum triglyceride, high-density lipoprotein, low-density lipoprotein, hemoglobin, and glycosylated hemoglobin were also assessed in experimental animals. The petroleum ether and ethanol extracts of C. fistula and the water-soluble fraction of ethanol extract were found to exhibit significant antihyperglycemic activity. The extracts, at the given doses, did not produce hypoglycemia in fasted normal rats, and the fraction exhibited weak hypoglycemic effect after 2 h of the treatment. Treatment of diabetic rats with ethanol extract and water-soluble fraction of this plant restored the elevated biochemical parameters significantly (P<0.05) to the normal level. No activity was found in the petroleum ether extract of the plant. Comparatively, the water-soluble fraction of ethanol extract was found to be more effective than the ethanol extract, and the activity was comparable with that of the standard, glibenclamide (5 mg/kg).

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