Notes1.8 g of 2-amino-2-methylpropan-l-ol in xylene solution (50 ml). After 7 days, the mixture was extracted to give 0.73 g of product: mp 79°; ir (Nujol) i/0h 3500, i-Nh 3200 cm"1; nmr (CDCls) CH3(4',4') 1.23, CH2(5') 3.55 ppm.Dd-Hydroxy-i'.f'-dimethylspirotSa-androstane-S^'-oxazoIidine)-3'-oxyl (3) was prepared as described above, using 0.73 g of crude amine and 0.53 g of m-chloroperbenzoic acid (oxidation time 5 hr). Thin layer chromatography gave 0.48 g of yellow product (yield 6>3%): mp 175-176°( methanol-water) (lit.2 mp 172-174°); uv (cyclohexane) 450 (e ~13); esr (CHC1S, M/ 1000) = 14.9 G. Spiro(5a-cholestane-3,5'-hydantoin) (7a). According to Maki,13 a mixture of 5a-cholestan-3-one (3.86 g), ammonium carbonate (5.7 g), and potassium cyanide (2 g) in 80% ethanol (150 ml) was heated at 57-58°for 10 days. The precipitate was filtered, washed with water, and dried to give 4 g of white powder (yield 87%); mp 274°; ir (KBr) yNH 3200, vc0 1780 and 1730 cm"1.The crude product (0.200 g) was extracted repeatedly with ethyl acetate to give 0.125 g of white powder 7a, mp 276°(lit.13 mp 273-274°), ir (KBr) identical with that of crude product.3a-Amino-5a-cholestane-3d-carboxylic Acid (8a). According to Maki,13 7a (0.125 g, mp 276°), sodium hydroxyde (5 g), andwater (5 ml) were heated for 1 hr with occasional addition of water. At the end of this time, a large amount of water was added and the mixture was filtered. The precipitate was dissolved byaddition of 70% sulfuric acid. The sulfate obtained was treated with concentrated ammonia to give 0.100 g (yield 87%) of white product 8a, mp 264°(lit.13 mp 262-264°).
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