Specificity of Staphylococcus aureus 209P cell wall hydrolysis by the L1 and L2-bacteriolytic enzymes from lysoamidase lytic complex was studied. L1-peptidase was shown to display both glycyl-glycine endopeptidase and N-acetylmuramyl-L-alanine amidase enzymatic activities on the S. aureus peptidoglycan molecule, whereas L2-peptidase acts as N-acetylmuramyl-L-alanine amidase.
Passage of light through a suspension is accompanied by a competition of processes of absorption and scattering for each individual particle. As a result, a hypochromism phenomenon (a decrease in the extinction coefficient) takes place. The hypochromism value increases with the growth of a particle sizes or its refractive index. Since Tyndall's light scattering in a suspension where the size of every particle is consid erably larger than the wavelength weakly depends on the wavelength, the absorption (or excitation) spectrum is almost uniformly attenuated. A simple method for determination of the true extinction coefficients of the absorption and excitation spectra of diluted suspensions which have no multiple light scattering is proposed. Experimental data on the spectra of hemoglobin in erythrocytes, actinomycin in DNA, and flavines in mito chondria are discussed.
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