“…The results obtained for the PRMC <0.22 μ are in good agreement with the results for the PRMC <0.45 μm [11] and confirm the earlier assumption that MC in specialized animal cells mainly arise not by fission or budding, but from germinal PRMC - particles smaller than 0.2 μm, containing DNA and a number of proteins.…”
Section: Resultssupporting
confidence: 89%
“…Thus, PRMC <0.2 μm do not have formed respiratory centers. That is why, in contrast to the PRMC with a diameter of 0.25–0.45 μm, which have respiration and even respiratory control [7,11], PRMC < 0.2 μm practically do not respire (data on oxygen consumption are not given).Fig. 3Absorption spectra of cytochromes of MC (1) and PRMC <0.2 μm (2) after destruction of the suspension particles by detergent (0.5% SDS).…”
Section: Resultsmentioning
confidence: 99%
“…PRMC were first isolated in our laboratory from the cells of the organs of animals (heart and liver) and some of their properties were studied [[7], [8], [9], [10], [11], [12], [13]]. Using hepatic PRMC with a diameter less than 0.45 μm, it was shown that these spherical particles are not MC fragments.…”
A number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes. Germinal PRMC contain in general the same proteins as MC. However, they have the reduced content of flavoproteins and zero cytochrome oxidase. Germinal PRMC, in contrast to MC, almost does not contain the “aging pigment” - lipofuscin. They have DNA; the DNA/protein ratio in them is much higher than in MC, i.e. they are poor in protein. The obtained results support the earlier assumption that MC in specialized animal cells can arise from germinal PRMC - particles smaller than 0.2 μm containing DNA. It is assumed that the DNA molecules enter to cytoplasm during degradation of old MC serves as a seed for the formation of PRMC (with the connection of nuclear DNA).
“…The results obtained for the PRMC <0.22 μ are in good agreement with the results for the PRMC <0.45 μm [11] and confirm the earlier assumption that MC in specialized animal cells mainly arise not by fission or budding, but from germinal PRMC - particles smaller than 0.2 μm, containing DNA and a number of proteins.…”
Section: Resultssupporting
confidence: 89%
“…Thus, PRMC <0.2 μm do not have formed respiratory centers. That is why, in contrast to the PRMC with a diameter of 0.25–0.45 μm, which have respiration and even respiratory control [7,11], PRMC < 0.2 μm practically do not respire (data on oxygen consumption are not given).Fig. 3Absorption spectra of cytochromes of MC (1) and PRMC <0.2 μm (2) after destruction of the suspension particles by detergent (0.5% SDS).…”
Section: Resultsmentioning
confidence: 99%
“…PRMC were first isolated in our laboratory from the cells of the organs of animals (heart and liver) and some of their properties were studied [[7], [8], [9], [10], [11], [12], [13]]. Using hepatic PRMC with a diameter less than 0.45 μm, it was shown that these spherical particles are not MC fragments.…”
A number of properties of the smallest (less than 0.2 μm) germinal proto-mitochondria (PRMC) from rat liver have been studied. These PRMC were obtained by filtering the light fraction of hepatic mitochondria (MC) through calibrated millipore membranes. Germinal PRMC contain in general the same proteins as MC. However, they have the reduced content of flavoproteins and zero cytochrome oxidase. Germinal PRMC, in contrast to MC, almost does not contain the “aging pigment” - lipofuscin. They have DNA; the DNA/protein ratio in them is much higher than in MC, i.e. they are poor in protein. The obtained results support the earlier assumption that MC in specialized animal cells can arise from germinal PRMC - particles smaller than 0.2 μm containing DNA. It is assumed that the DNA molecules enter to cytoplasm during degradation of old MC serves as a seed for the formation of PRMC (with the connection of nuclear DNA).
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