Cellulase and xylanase were covalently immobilized on chitosan magnetic particles. They were employed as catalysts to produce reducing sugar from coconut husk individually and simultaneously. Fourier Transform Infrared Spectroscopy (FT-IR) and Bradford's protein analysis confirmed that the enzymes were covalently immobilized on the support. Cellulase from Aspergillus niger and Trichoderma reesei immobilized on chitosan magnetic microparticles yielded 0.352 g/L and 0.316 g/L of reducing sugar respectively, while immobilized xylanase from Trichoderma longibachterium yielded 0.432 g/L. The simultaneous use of cellulase and xylanase produced 0.8034 g/L of sugar, and the addition of glutaraldehyde as a cross-linking agent increased the amount of reducing sugar. Enzyme could maintain its activity at 91% for up to five cycles. Using nanosized particles resulted in a sugar yield of 0.49 g/l and 54.7 % of its activity maintained after five cycles.

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