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Electrochemical cell-based biosensors have been showing increasing interest within the last 15 years, with a large number of reports generally dealing with the sensors’ sensitivity, selectivity, stability, signal-to-noise ratio, spatiotemporal resolution, etc. However, only a few of them are now available as commercial products on the market. In this review, technological advances, current challenges and opportunities of electrochemical cell-based biosensors are presented. The article encompasses emerging studies, mainly focusing on the last five years (from 2016 to mid 2021), towards cell-based biological field-effect devices, cell-based impedimetric sensors and cell-based microelectrode arrays. In addition, special attention lies on recent progress in recording at the single-cellular level, including intracellular monitoring with high spatiotemporal resolution as well as integration into microfluidics for lab-on-a-chip applications. Moreover, a comprehensive discussion on challenges and future perspectives will address the future potential of electrochemical cell-based biosensors.

A light‐addressable potentiometric sensor (LAPS) is a field‐effect‐based (bio‐) chemical sensor, in which a desired sensing area on the sensor surface can be defined by illumination. Light addressability can be used to visualize the concentration and spatial distribution of the target molecules, e.g., H+ ions. This unique feature has great potential for the label‐free imaging of the metabolic activity of living organisms. The cultivation of those organisms needs specially tailored surface properties of the sensor. O2 plasma treatment is an attractive and promising tool for rapid surface engineering. However, the potential impacts of the technique are carefully investigated for the sensors that suffer from plasma‐induced damage. Herein, a LAPS with a Ta2O5 pH‐sensitive surface is successfully patterned by plasma treatment, and its effects are investigated by contact angle and scanning LAPS measurements. The plasma duration of 30 s (30 W) is found to be the threshold value, where excessive wettability begins. Furthermore, this treatment approach causes moderate plasma‐induced damage, which can be reduced by thermal annealing (10 min at 300 °C). These findings provide a useful guideline to support future studies, where the LAPS surface is desired to be more hydrophilic by O2 plasma treatment.