Differential chemical imaging of extracellular acidification within microfluidic channels using a plasma-functionalized light-addressable potentiometric sensor (LAPS)

Özsoylu, Dua; Kızıldağ, Sefa; Schöning, Michael J.; Wagner, Torsten

doi:10.1016/j.phmed.2020.100030

Cited by 9 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Can be used as imaging devices allowing to visualize the ion concentration as a two-dimensional map [198] Potentiometric sensor pH is determined from the voltage measured between a reference electrode and a sensitive electrode obtained exploiting metal oxides sensitivity to H+…”

Section: Light Addressable Potentiometric Sensor (Laps)mentioning

confidence: 99%

“…Indeed, these sensors have been used in microfluidic systems: LAPS integrated with a PDMS chamber have been used for real-time pH monitoring of the medium where hepatoma HepG2 cells were cultured and treated with doxorubicin in order to assess the extracellular acidification rate [ 209 ]; and, in a microphysiometer for pH monitoring to track the metabolism of human breast cancer cells [ 210 ]. Furthermore, by cultivating adherent CHO-K1 on the LAPS surface, it was possible to image the extracellular acidification [ 198 ]. The cell’s environment’s pH can be controlled through potentiometric measures by placing the electrodes in a microfluidic device [ 211 ].…”

Section: Advantages Of Microfluidic-chip Technology For Evolving Cmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

Duzagac

Saorin

Memeo³

et al. 2021

Cancers

View full text Add to dashboard Cite

Organ-like cell clusters, so-called organoids, which exhibit self-organized and similar organ functionality as the tissue of origin, have provided a whole new level of bioinspiration for ex vivo systems. Microfluidic organoid or organs-on-a-chip platforms are a new group of micro-engineered promising models that recapitulate 3D tissue structure and physiology and combines several advantages of current in vivo and in vitro models. Microfluidics technology is used in numerous applications since it allows us to control and manipulate fluid flows with a high degree of accuracy. This system is an emerging tool for understanding disease development and progression, especially for personalized therapeutic strategies for cancer treatment, which provide well-grounded, cost-effective, powerful, fast, and reproducible results. In this review, we highlight how the organoid-on-a-chip models have improved the potential of efficiency and reproducibility of organoid cultures. More widely, we discuss current challenges and development on organoid culture systems together with microfluidic approaches and their limitations. Finally, we describe the recent progress and potential utilization in the organs-on-a-chip practice.

show abstract

Section: Light Addressable Potentiometric Sensor (Laps)mentioning

confidence: 99%

Section: Advantages Of Microfluidic-chip Technology For Evolving Cmentioning

confidence: 99%

Microfluidic Organoids-on-a-Chip: Quantum Leap in Cancer Research

Duzagac

Saorin

Memeo³

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…Before seeding the cells, the microfluidic channels were sterilized by flushing them with 70% ethanol for one hour. Cell culturing was performed as mentioned in the literature [11]. 40000 cells/cm 2 were seeded into each channel.…”

Section: Cell Culturing and Cryopreservationmentioning

confidence: 99%

“…This way, the chip was connected to the self-developed LAPS measurement set-up. Extracellular acidification of the cells was monitored as previously reported in the literature [11,18].…”

Section: Sensor Signal Measurement After Cryopreservationmentioning

confidence: 99%

“…This feature represents a remarkable advantage in achieving spatial resolution in biosensing applications [9,10]. For example, using a LAPS chip that has a pH-sensitive transducer layer, a desired sensing point on the sensor surface can be addressed to measure the extracellular acidification (EA) of the cells above that region [11]. Moreover, a larger area can be addressed point-by-point in a scanning manner.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation