The cultivation of pineapple contributes 1.6% of the gross Ivorian national product (GDP). However, this crop is facing a severe production crisis due to the aging of the orchards. Revising this sector requires the rejuvenation of orchards with healthy and improved planting material. This work was conducted to study the conditions for the efficient in vitro production of restorative pineapple planting material by somatic embryogenesis. The effects of seven culture media consisting of a different combination of nitrogen sources (casein hydrolyzate, glutamine, and glycine), cytokinins (kinetin or BAP), and auxins (2,4-D or picloram) were tested on somatic embryos induction and maturation in pineapple. Results of the study revealed that EIM1 (EIM added with 3 mg.L-1 picloram, 0.05 mg.L-1BAP, 2 mg.L-1 glycine, 1000 mg.L-1glutamine, 100 mg.L-1casein hydrolyzate) and EIM5 (EIM added with 2 mg.L-1glycine, 100 mg.L-1casein hydrolyzate, 0.2 mg.L-1kinetin) media induced the highest numbers of embryogenic cells, i.e., 154 and 149 cells respectively. Further, the EIM5 medium was more embryogenic, with the most significant number of mature embryos (66 mature embryos), and allowed the observation of all embryonic maturation stages. Embryogenic cell induction in pineapple is thought to be controlled by a low NH4+/NO3- ratio in interactions with phytohormones. In the presence of 2,4-D, embryogenic cell maturation was improved by kinetin addition to the culture medium containing glycine and casein hydrolyzate.
Ginger (Zingiber officinale Rosc.) is a spice, considered as a food medicine because of its numerous beneficial actions on health. However, its production faces several constraints, including the lack of efficient planting material. This is a limiting factor for industrial ginger production. The present study therefore aimed at developing an effective in vitro regeneration protocol for ginger. First, three disinfecting agents (sodium hypochlorite, calcium hypochlorite and mercury chloride) were tested. Then, different combinations of naphthalene acetic acid (0.5 mg/L NAA) and/or benzyl amino purine (1, 3 and 5 mg/L BAP) were evaluated on in vitro shoots regeneration. The results revealed that 3.6% sodium hypochlorite, used for 20 min, induced the best disinfection rates (100%) and healthy buds (84.66%). Furthermore, this study showed that the tested hormonal combinations significantly influenced shoots proliferation in ginger. However, the Proliferation Medium 4 (PM4) [Murashige and Skoog medium including vitamin B5 (MSB) + 0.5 mg/L of Naphthalene Acetic Acid (ANA) + 5 mg/L of Benzyl Amino Purine (BAP)] was the most effective. It induced the highest average number of shoots (22.83 shoots) with an induction rate of 80.50%. As a result of this study, 3.6% sodium hypochlorite used for 20 min and MP4 medium (MSB + 0.5 mg/L ANA + 5 mg/L BAP) were selected for in vitro ginger regeneration.
A technique was developed to induce callus formation and differentiation using leaf, petiole and root explants from Bambara groundnut [Vigna subterranea (L.) Verdc.]. The combinations and concentrations of different growth regulators were shown to be critical factors in the frequency of callus formation, as well as, in the potential for callus differentiation. It was established that the best responses in callus induction (75 %) were obtained from petiole on a MS (Murashige and Skoog, 1962) basal medium, supplemented with 3 -5 mg/l N-benzylaminopurine (BAP) + 0.5 mg/l α-naphthaleneacetic acid (NAA). Callus growth (GI) was more significant with Ci1 (3.52) and Ci4 (2.76) landraces, while root formation was especially observed with the Ci3 (24.81 %) landrace. Here, for the first time, induction of embryogenic and organogenic callus from petiole, leaf and root of Vigna subterranea (L.) was reported. Both adventitious buds and somatic embryos were initiated after 3 months of subculture on a callus induction medium containing 3-5mg/l BAP + 0.1 -0.5 mg/l ANA. Somatic embryogenesis (50 -68 %) occur more frequently than organogenesis (11 -27 %).Keywords : Callus, bambara groundnut (Vigna subterranea L.), somatic embryogenesis, organogenesis. Ci1 (3,52) et Ci4 (2,76) RESUME FACTEURS INFLUENÇANT L'INDUCTION ET LA DIFFERENCIATION DE CALS Un protocole a été développé pour induire la formation de cals, à partir des explants de feuille, de pétiole et de racine chez le pois de bambara [Vigna subterranea (L.)]. Les combinaisons et les concentrations de différents régulateurs de croissance se sont avérées être des facteurs déterminants dans la fréquence d'induction des cals, de même que pour le potentiel de différenciation des cals. Les résultats montrent également que l'induction des cals a été plus importante avec le pétiole (75 %) sur le milieu de base MS (Murashige et Skoog, 1962) supplémenté avec 3 -5 mg/l de la N-benzylaminopurine (BAP) + 0,5 mg/l de l'acide α-naphthalène acétique (ANA). La croissance des cals (IC) a été plus importante avec les écotypes 16M. KONE et al.
The study aimed at controlling the mycelial growth and germination of Mycosphaerella fijiensis in Petri dish by using three plant defense stimulators. Approximately, 2 mL of each stimulator (Vacciplant®, Callel® and calliete®) were added to the different M. fijiensis culture media. The results obtained show that Vacciplant® and Callel® promote the in vitro growth of M. fijiensis contrary to calliete® which inhibits it. Indeed, it causes total mortality (100%) of conidia at the sporulation stage.
In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%). So, carbone sources, hormone, and their concentration affect significantly callus induction. The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucose. The induced-callus obtained were friable and of high quality. The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it. The present study may provide an efficient protocol to improve the callogenesis responses by adjusting the carbohydrates, the hormonal regime, and the type of explant to be cultured for a high-frequency callus induction of the Y331B-R5 cotton cultivar.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.