In vitro culture of Bambara groundnut [<i>Vigna subrerranea</i> (L.) Verdc., Favaceae ]: Effect of plant growth regulators, explant type and genotype on callus induction and differentiation
Abstract:A technique was developed to induce callus formation and differentiation using leaf, petiole and root explants from Bambara groundnut [Vigna subterranea (L.) Verdc.]. The combinations and concentrations of different growth regulators were shown to be critical factors in the frequency of callus formation, as well as, in the potential for callus differentiation. It was established that the best responses in callus induction (75 %) were obtained from petiole on a MS (Murashige and Skoog, 1962) basal medium, suppl… Show more
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