ResumoTransferibilidade de primers heterólogos e acesso ao polimorismo de loci microssatélites em maracujazeiro-do-sono (Passilora setacea DC). Pares de primers que acessam loci microssatélites, inicialmente construídos a partir do genoma de Passilora edulis Sims lavicarpa e P. alata, foram testados quanto à sua capacidade de acessar loci microssatélites em indivíduos de maracujazeiro-do-sono (P. setacea). Sete dos trinta e um pares de primers testados foram capazes de acessar polimorismo de DNA no genoma dessa espécie silvestre de Passilora, ao avaliar seis populações naturais, localizadas em área de transição entre os biomas Caatinga e Cerrado, no estado da Bahia. O número de alelos/loci foi baixo, oscilando de um a quatro. A heterozigosidade média observada por locus em todas as populações variou de 0,13 a 0,40. Houve transferência dos pares de primers microssatélites heterólogos do gênero Passilora para maracujazeiro-do-sono, constituindo um novo conjunto de primers que acessam locus codominante aleatório nessa espécie, útil para ins de conservação e pré-melhoramento do maracujazeiro-do-sono. Palavras-chave: Diversidade genética; Maracujazeiro-do-sono; Simple sequence repeat; Transferibilidade
AbstractPrimer pairs that access microsatellite loci, initially constructed through the genome of Passilora edulis Sims lavicarpa and P. alata, were tested concerning their ability to access microsatellite loci in "somnus" passion fruit tree (P. setacea) individuals. Seven out of the thirty one primer pairs tested were able to access DNA polymorphism in the genome of this wild Passilora species, by evaluating six natural populations, located in a transition area between the biomes Caatinga and Cerrado, in the state of Bahia, Brazil. The number of alleles/ loci was small, oscillating from 1 to 4. The average heterozygosity observed per locus in all populations ranged from 0.13 to 0.40. There was transference of heterologous microsatellite primer pairs from the Passilora genus to "somnus" passion fruit tree, constituting a new set of primers that access random co-dominant locus in this species, useful for conservationist purposes and pre-improvement of "somnus" passion fruit tree.
On farm conservation of agrobiodiversity in family farms in Jequié, Bahia State, BrazilThe Caatinga is a priority area for biodiversity conservation in Brazil. In these areas there are many communities of farmers that maintain traditional agricultural practices which are vital for on farm conservation. The aim of this study was a survey on the socioeconomic and cultural profile of stallholders, identifying species and managements in their farms that favor on farm conservation of the diversity of cultivated species. The influence of the area of cultivated land on the richness and abundance of plants was analyzed. The survey was carried out through visits to street markets and family farmer units located in the municipality of Jequié -BA, Northeastern Brazil. Twenty-eight species were cited for food utilization, belonging to 17 families, totalling 75 etnovarieties. The influence of the area of cultivated land on the species richness showed low correlation (r = 0.29, p < 0.01), but showed a strong correlation (r = 0.69, p <0.01) on species abundance. Most farmers have little formal schooling and low income. The occurrence of on farm conservation was detected in the studied areas.
ResumoEste trabalho tem por objetivo familiarizar o público-alvo com termos da comunicação científica em biologia reprodutiva com ênfase em polinização, sendo fundamental para o profissional que pretende elaborar e publicar trabalhos técnico-científicos nesta importante área de pesquisa. Neste sentido, é apresentado um glossário de termos usuais em revistas científicas e em órgãos de pesquisa diversificados.
AbstractTerminology of pollination biology applied to fruit culture. The aim of this work was to familiarize a target audience with the terminology of scientific communication in reproductive biology with emphasis on pollination in fruit trees. This is fundamental to the professional who aims to develop and publish technical and scientific writings in this important area of research. To this end, a glossary of the usual terms employed in scientific journals and research institutes is presented.
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