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ft has been known for many years that muscle contraction is accompanied by appearance of equivalent amounts of inorganic phosphate and free creatine, creatinephosphate disappearing. Until the work of Lohmann [1934] using muscle extract, this process was regarded as an enzymic hydrolysis of creatinephosphate, going on with liberation of energy. But Lohmann showed that the breakdown of creatinephosphate is a composite process, requiring the presence in the extract of adenylic acid and adenosinetriphosphatase:(I) 2 creatinephosphate + adenylic acid -* 2 creatine + adenosinetriphosphate adenosinetriphosphate -+ adenylic acid + 2H3PO4 .

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