Uptake of 3H-thymidine by resting cells of Pediococcus cerevisiae was found to be energy-and temperature-dependent. The pH optimum was between 6.5 and 8.0, and after 2 min of incubation most of the radioactivity was found in the deoxyribonucleic acid (DNA) fraction. lodoacetate at a concentration of 10-2 M caused a 50% inhibition of uptake. Preincubation of resting cells for 10 min with folinate