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1 Three human adenocarcinoma cell lines, , Col-6 and Col-1 have been studied as con¯uent epithelial layers able to transport ions vectorially in response to basolateral vasoactive intestinal polypeptide (VIP) and pancreatic polypeptides (PP). 4 GR231118 (1 mM) had no PP-like e ects. In Col-24 and Col-1, GR231118 signi®cantly attenuated bPP (30 nM) or hPP (100 nM) responses, but it did not alter bPP responses in Col-6. BIBP3226 and GR231118 both inhibited Y 1 -mediated responses which were only present in Col-6. 5 RT ± PCR analysis con®rmed the presence of hY 4 receptor mRNA in Col-24 and Col-1 epithelia but a barely visible hY 4 product was observed in Col-6 and we suggest that an atypical Y 4 receptor is expressed in this cell line.