Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene

O’Keefe, Denise S.; Su, Sai L.; Bacich, Dean J.; Horiguchi, Yutaka; Luo, Ying; Powell, C. Thomas; Zandvliet, Dorothea W.J.; Russell, Pamela J.; Molloy, Peter L.; Nowak, Norma J.; Shows, Thomas B.; Mullins, Cami; Haar, Raymond A. Vonder; Fair, William R.; Heston, Warren D.W.

doi:10.1016/s0167-4781(98)00200-0

Cited by 176 publications

(119 citation statements)

References 42 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…Restriction mapping and sequencing confirmed identity to the GCP II gene (O'Keefe et al, 1998 GenBank Accession No. AF007544; Good, 1999, GenBank Accession No.…”

Section: Human Library Screening and Analysismentioning

confidence: 86%

“…Previous studies reported that 2 Kb and 1.2 Kb of the 5′ promoter of the PSMA gene were effective in driving reporter gene expression in prostate-derived cell lines and non-prostate cell lines but not in CNS-derived cell lines (O'Keefe et al, 1998;Good et al, 1999). However, GCP II is expressed in the brain, with particularly high abundance in astrocytes (Berger et al, 1999;Sacha et al, 2007).…”

Section: Discussionmentioning

confidence: 98%

“…Restriction mapping and sequencing showed that the clone shared sequence identity with the human PSMA gene (O'Keefe et al, 1998;Good et al, 1999) and spanned the 3′ extreme of exon 1 through upstream flanking regions in the 5′ untranscribed sequences of GCP II gene. Based on the published cDNA sequence for the PSMA gene (Israeli et al, 1993), the transcription start site should be located at nt 5290 and the translation start site (ATG) at nt 5551.…”

Section: Discussionmentioning

confidence: 99%

“…O'Keefe et al (1998), reported that a 1.2 Kb portion of the 5′ region of the GCP II gene was able to drive reporter gene expression in prostate but not breast-derived cell lines (O'Keefe et al, 1998). A 2-Kb human genomic fragment containing the 5′ untranscribed region of the GCP II gene was identified in prostate and non-prostate cell lines (Good et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Promoter analysis of human glutamate carboxypeptidase II

et al. 2007

View full text Add to dashboard Cite

The expression of glutamate carboxypeptidase II (GCP II) is reduced in selective brain regions in schizophrenic patients. To investigate transcriptional mechanisms regulating the human GCP II gene, a 3460 bp DNA fragment comprised of the proximal 3228 bp of 5′ untranscribed sequence and first 232 bp of 5′ UTR portion of this gene was cloned into the mammalian luciferase reporter gene vector pGL3-Basic. Transfection assays in human astrocyte-derived SVG and human prostate tumorderived LNCaP cells demonstrated that constructs with 3460, 1590 and 761 bp portions of 5′ region of human GCP II gene were able to drive the luciferase reporter gene. Additional deletion constructs showed that in the SVG cell line, constructs with 511 and 411 bp of GCP II gene fragments yielded highest transcriptional activity, with declining activity upon further removal of 5′ sequences. Fifteen bp of the promoter 5′ to a 225 bp GCP II fragment were essential for luciferase expression. Thus, in the SVG cells, the proximal 240 bp of the human GCP II promoter (232 bp of the 5′ UTR and 8 bp of 5′ untranscribed sequences) may represent the core promoter. Further, while a LyF-1 site lies within and overlaps a transcription start site in the 15 bp sequence, site-directed mutagenesis shows that LyF-1 is not the transcription iniatiator for the "TATA and CAAT" box lacking GCP II gene in the SVG cells. Finally, pattern differences in GCP II gene promoter expression in SVG and LNCaP cells suggest that sequences beyond 240 bp may be important for tissue-specific GCP II expression.

show abstract

“…Restriction mapping and sequencing confirmed identity to the GCP II gene (O'Keefe et al, 1998 GenBank Accession No. AF007544; Good, 1999, GenBank Accession No.…”

Section: Human Library Screening and Analysismentioning

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Promoter analysis of human glutamate carboxypeptidase II

et al. 2007

View full text Add to dashboard Cite

show abstract

“…However, this has now been resolved. 25,52 Detailed genetic analysis has allowed localisation of the FOLH1 gene to the 1lp11.2 chromosomal region at 62.5 cM, with a related FOLH2 gene at 11q14. Interestingly there appears to be extensive genetic duplication, over at least 500 kb, in these two regions.…”

Section: Discussionmentioning

confidence: 99%