Prostate-specific membrane antigen (FOLH1): recent advances in characterising this putative prostate cancer gene

Maraj, BH; Markham, AF

doi:10.1038/sj.pcan.4500325

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…This region has been suggested as a tumor suppressor locus for liver cancer (Ricketts et al 2002) and is involved in translocation in lymphoma (Cuneo et al 2001). One end of this region coincides with an insertion of prostate surface antigen and prostate cancer prognostic marker gene FOLH1 (Maraj and Markham 1999) found in mouse chromosome 7qD3. This gene together with its flanking newly evolved human-specific genes, FGCP, is located at the breakpoint joining mouse 2qE1 and 2qD.…”

Section: Protection Of Tspri Dna Fragments From Exonuclease III Digesmentioning

confidence: 99%

Genome-wide mapping and characterization of hypomethylated sites in human tissues and breast cancer cell lines

Shann¹,

Cheng²,

Chiao³

et al. 2008

Genome Res.

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We have developed a method for mapping unmethylated sites in the human genome based on the resistance of TspRI-digested ends to ExoIII nuclease degradation. Digestion with TspRI and methylation-sensitive restriction endonuclease HpaII, followed by ExoIII and single-strand DNA nuclease allowed removal of DNA fragments containing unmethylated HpaII sites. We then used array comparative genomic hybridization (CGH) to map the sequences depleted by these procedures in human genomes derived from five human tissues, a primary breast tumor, and two breast tumor cell lines. Analysis of methylation patterns of the normal tissue genomes indicates that the hypomethylated sites are enriched in the 5Ј end of widely expressed genes, including promoter, first exon, and first intron. In contrast, genomes of the MCF-7 and MDA-MB-231 cell lines show extensive hypomethylation in the intragenic and intergenic regions whereas the primary tumor exhibits a pattern between those of the normal tissue and the cell lines. A striking characteristic of tumor cell lines is the presence of megabase-sized hypomethylated zones. These hypomethylated zones are associated with large genes, fragile sites, evolutionary breakpoints, chromosomal rearrangement breakpoints, tumor suppressor genes, and with regions containing tissue-specific gene clusters or with gene-poor regions containing novel tissue-specific genes. Correlation with microarray analysis shows that genes with a hypomethylated sequence 2 kb up-or downstream of the transcription start site are highly expressed, whereas genes with extensive intragenic and 3Ј untranslated region (UTR) hypomethylation are silenced. The method described herein can be used for large-scale screening of changes in the methylation pattern in the genome of interest.

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Section: Protection Of Tspri Dna Fragments From Exonuclease III Digesmentioning

confidence: 99%

Genome-wide mapping and characterization of hypomethylated sites in human tissues and breast cancer cell lines

Shann¹,

Cheng²,

Chiao³

et al. 2008

Genome Res.

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“…In the present study, we screened the normal and cancer tissue-specific proteins among 14 different tissues. Some well-known tissue-specific proteins were also identified, such as ESR1 in breast cancer, shown to be associated with high grade and high proliferation (Moelans et al, 2010), CDX2 in colorectal cancer, used as a highly sensitive and specific marker of adenocarcinomas of intestinal origin (Werling et al, 2003), KLK2 and KLK3 in prostate cancer, utilized as biomarkers (Penney et al, 2011), and ACPP and FOLH1, identified as prostate-specific cancer proteins (Maraj and Markham, 1999). Of the 14 tissues examined, thyroid cancer tissue has the highest number of cancer tissue-specific proteins (103), including the well-known thyroid specific proteins TG and TPO (González et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Novel bioinformatic identification of differentially expressed tissue-specific and cancer-related proteins from the Human Protein Atlas for biomarker discovery

2015

Genet. Mol. Res.

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ABSTRACT. Identification of cancer-associated and tissue-specific proteins is important for research on carcinogenesis mechanisms and biomarker discovery. Here we performed a new strategy to identify candidate cancer proteins by mining immunohistochemistry protein profiles. Proteins with quantitative values from 14 normal tissues and their corresponding cancer tissues were compared and analyzed using bioinformatics. The final results included identification of tissue-specific proteins and differentially expressed proteins in different cancer types that are primarily involved in energy metabolism and cell invasion. From the tissue-specific proteins, secreted and membrane proteins were further screened and functionally clustered. These primarily belonged to the gene families of endogenous ligands, cluster of differentiation molecules, and solute carriers, and were mainly involved in the processes of cell motility, hormone metabolism, adhesion, and transport. Further studies are warranted to validate the candidates identified herein and substantiate the suggested enriched functions. The results 4557-4565 (2015) from this study might provide a reliable resource to study underlying carcinogenesis mechanisms and discover potential cancer targets for the development of therapeutic targets and of early diagnosis and disease response markers.

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ImmunoPET for prostate cancer in the PSMA era: do we need other targets?

Filippi

Evangelista²,

Sathekge³

et al. 2022

Clin Transl Imaging

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Prostate-specific membrane antigen (FOLH1): recent advances in characterising this putative prostate cancer gene

Cited by 5 publications

References 24 publications

Genome-wide mapping and characterization of hypomethylated sites in human tissues and breast cancer cell lines

Genome-wide mapping and characterization of hypomethylated sites in human tissues and breast cancer cell lines

Novel bioinformatic identification of differentially expressed tissue-specific and cancer-related proteins from the Human Protein Atlas for biomarker discovery

ImmunoPET for prostate cancer in the PSMA era: do we need other targets?

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