Periodontitis, characterized by gingival inflammation and resorption of alveolar bone, is classically described as a bacterially induced chronic inflammatory disease of the periodontium (Darveau, 2010). The initial manifestation of periodontal disease chiefly includes gingivitis manifesting as gingival redness, swelling, and bleeding. With disease progression, periodontal attachment structures are destructed to varying degrees, manifesting as alveolar bone resorption and gingival recession (de Paiva Goncalves et al., 2018). As a specialized cellular connective tissue, the periodontal ligament (PDL) surrounds and anchors the teeth to the alveolar bone and periodontal ligament cells (PDLCs) retain capacity of promoting alveolar bone regeneration (Ivanovski et al., 2006).Porphyromonas gingivalis lipopolysaccharide (P. g. LPS) is believed to play a significant role in the pathogenesis of periodontitis (Liu et al., 2018;Yang et al., 2021) and has been widely applied to induce periodontitis for experimental purposes (Kim et al., 2019;
In order to explore the segmented relationship between the segmented rating results of Baijiu and the segmented characteristics of alcohol content and cumulative flow in the distillation process and to verify whether different liquor categories can achieve rating through the segmented characteristics of alcohol content and cumulative flow, in view of this, combined with the Industrial Internet of Things (IIoT) technology and online detection and analysis of alcohol and cumulative traffic, its accuracy can reach ±0.5%, ±1%, with the integration of Baijiu categories, sources, liquor characteristic conditions, and other multisource data, to achieve Baijiu segmented rating data reconstruction, the use of standard error and standard deviation as evaluation indicators, quantification of distilled liquor alcohol, and cumulative flow segmented characteristics to form a liquor rating strategy, so as to use the Arduino platform control motor to achieve automatic grading of Baijiu. Experiments show that the relative error between automatic rating and manual rating is less than 10%, which shows that automatic rating can be better applied to the actual brewing process. It provides a solution for the digitization and standardization of Baijiu grading.
The current study aimed to determine the expression of carnitine palmitoyltransferase 1A (Cpt1a) in the lung tissue of chronic obstructive pulmonary disease (COPD) patients and its correlation with lung function. An increase in Cpt1a expression improved lung function in patients with COPD by inhibiting apoptosis and the inflammatory response of lung endothelial cells. Lung tissues of 20 patients with COPD and 10 control patients were collected, their Cpt1a expression was determined by western blotting and apoptosis and inflammation were assessed by haematoxylin-eosin staining, TUNEL assay and ELISA. Mice with knockout or overexpression of Cpt1a were constructed by lentivirus in vivo. A COPD model was induced by cigarette smoke and the role of Cpt1a in COPD was determined in vivo and in vitro. Cpt1a expression was positively correlated with lung function and negatively correlated with apoptosis and inflammation. Patients with COPD with higher expression of Cpt1a in lung tissues had improved lung function indices and lung tissue morphology with less apoptosis and decreased inflammatory response. Compared with the control group, COPD mice with Cpt1a knockdown had aggravated lung dysfunction and increased lung inflammation and apoptosis. Overexpression of Cpt1a alleviated lung dysfunction and reduced inflammatory response and apoptosis of lung tissues in COPD mice. Pulmonary microvascular endothelial cells of mice were isolated in vitro and the results were consistent with the findings obtained in vivo. In conclusion, the clinical, in vivo and in vitro data confirmed for the first time that Cpt1a alleviated lung dysfunction of patients with COPD by inhibiting apoptosis of endothelial cells and inflammatory responses.
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