Background and aim
Deleterious cutaneous tissue damages could result from exposure to thermal trauma, which could be ameliorated structurally and functionally through therapy via the most multipotent progenitor bone marrow mesenchymal stem cells (BM-MSCs). This study aimed to induce burns and examine the effect of BM-MSCs during a short and long period of therapy.
Material and methods
Ninety albino rats were divided into three groups: group I (control); group II (burn model), the animals were exposed to the preheated aluminum bar at 100°C for 15 s; and group III (the burned animals subcutaneously injected with BM-MSCs (2×106 cells/ ml)); they were clinically observed and sacrificed at different short and long time intervals, and skin samples were collected for histopathological and immunohistochemical examination and analysis of different wound healing mediators via quantitative polymerase chain reaction (qPCR).
Results
Subcutaneous injection of BM-MSCs resulted in the decrease of the wound contraction rate; the wound having a pinpoint appearance and regular arrangement of the epidermal layer with thin stratum corneum; decrease in the area percentages of ADAMs10 expression; significant downregulation of transforming growth factor-β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), metalloproteinase-9 (MMP-9), and microRNA-21; and marked upregulation of heat shock protein-90α (HSP-90α) especially in late stages.
Conclusion
BM-MSCs exhibited a powerful healing property through regulating the mediators of wound healing and restoring the normal skin structures, reducing the scar formation and the wound size.
Leukemia is an incurable disease; it exhibits strong resistance to chemotherapy and other therapies, its seriousness is not only in this fact but also, it represents the most common childhood cancer, and mortality. In this study, the cytotoxic of amygdalin (AMG) against the cell line of human monocytic leukemia (THP-1) was recoded in attempting for providing a cure for such monster cancerous type, be siding determining other pharmacological effects as anti-microbial activity. The cells were exposed to Vit b17 for 24 hr at 37°C at different concentrations, the cytotoxic effect was determined via the MTT assay. The cells and the supernatant were collected for analyzing the oxidant/antioxidant status, apoptotic markers, and anti-microbial activity. The results showed a marked anti-proliferative cytotoxic effect of Vit b17 which is concentration and time-dependent, the lipid peroxidation content was significantly decreased while the total thiol was significantly increased in the treated cell line, significant up-regulation of the transcript level of Caspase-3 (Cas-3) and Bcl-2-associated X protein (Bax) and down-regulation of B-cell lymphoma 2 (BCL-2). Furthermore, The bacterial activity was detected via Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Disc Diffusion assays, while the antifungal evaluation was done by the Minimum Fungicidal Concentration (MFC). Antimicrobial experiments revealed that AMG exerted potent, broad-spectrum antimicrobial effects towards a diversity of dangerously infected pathogens. In conclusion, the prevailing research suggests that AMG is an effective antibacterial substance against a wide range of microbial pathogens that cause disease, in addition to its anticancer activity against human monocytic leukemia.
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