Establishment of amygdalin antitumor efficacy against human acute monocytic leukemia cell line (THP‐1) with antimicrobial investigations, in vitro Study

Abstract

Leukemia is an incurable disease; it exhibits strong resistance to chemotherapy and other therapies, its seriousness is not only in this fact but also, it represents the most common childhood cancer, and mortality. In this study, the cytotoxic of amygdalin (AMG) against the cell line of human monocytic leukemia (THP-1) was recoded in attempting for providing a cure for such monster cancerous type, be siding determining other pharmacological effects as anti-microbial activity. The cells were exposed to Vit b17 for 24 hr at 37°C at different concentrations, the cytotoxic effect was determined via the MTT assay. The cells and the supernatant were collected for analyzing the oxidant/antioxidant status, apoptotic markers, and anti-microbial activity. The results showed a marked anti-proliferative cytotoxic effect of Vit b17 which is concentration and time-dependent, the lipid peroxidation content was significantly decreased while the total thiol was significantly increased in the treated cell line, significant up-regulation of the transcript level of Caspase-3 (Cas-3) and Bcl-2-associated X protein (Bax) and down-regulation of B-cell lymphoma 2 (BCL-2). Furthermore, The bacterial activity was detected via Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Disc Diffusion assays, while the antifungal evaluation was done by the Minimum Fungicidal Concentration (MFC). Antimicrobial experiments revealed that AMG exerted potent, broad-spectrum antimicrobial effects towards a diversity of dangerously infected pathogens. In conclusion, the prevailing research suggests that AMG is an effective antibacterial substance against a wide range of microbial pathogens that cause disease, in addition to its anticancer activity against human monocytic leukemia.