SUMMARY The objective of this study was to evaluate the performance of male colonial chickens fed diet supplemented with commercial probiotic (Calsporin ®). A total of 210 male chicks Label Rouge lineage were used and they were raised in experimental shed up to 30 days old. These birds had free access to the pickets of Tifton-85, from 31-90 days of age. The experimental design was completely randomized, two treatments, one containing feed supplemented with probiotic Bacillus subtillis (300 g t-1 Bacillus subtillis 1×109 UFCg-1) and another one without it, with ten replications per treatment, consisting of 21 chickens/replication. The mean of body weight (BW), weight gain (WG), feed intake (FI), feed conversion (FC), mortality and viability were evaluated. The data were submitted to variance analysis and the averages of the treatments were compared by the F-test at 5% of significance. The addition of probiotic (300 g t-1 Bacillus subtillis 1×109 UFCg-1) in the diet of Label Rouge broilers did not interfere in the MW, WG, and FC variables in the total period from 1 to 90 days (P>0.05); the MFI variable differed (P <0.05), with higher consumption in chickens receiving the diet with the probiotic. The inclusion of probiotic in the wild-type chicken ration did not improve the productive performance of poultries possibly due to a low challenge by pathogenic microorganisms in the environments in which they were raised.

The objective of this study is to evaluate the effects of in-egg inoculation with sources of conjugated linoleic acid (CLA) and lauric acid (AL), going by the weight of lymphoid organs and the biochemical profile of the blood of cut quails, from one to 35 days of age. We used 360 quails distributed in a completely randomized design, in six treatments and six replicates of 10 birds per plot. Corn oil (OM) was used to dilute the CLA and AL. The experimental treatments were: healthy eggs (control); eggs inoculated with OM thinner; eggs inoculated with CLA 120 mg / 50 mL OM; eggs inoculated with CLA 240 mg / 50 mL OM; eggs inoculated with AL 60 mg / 50 mL OM and eggs inoculated with AL 90 mg / 50 mL OM. The results were analyzed through Program R at the level of 5% significance. To verify the normal distribution of errors, the Shapiro-Wilk normality test was applied. At 21 days, an effect of p < 0.05 was observed for the total blood cholesterol content, inoculations with CLA reduced, even as AL increased, regardless of the level. At 35 days, it was found (p < 0.05) that the levels of total cholesterol and AST in the blood decreased with inoculations of CLA and AL at the lower levels, compared to those that did not receive the inoculations. The CLA and AL levels supplemented via egg inoculations did not interfere with the weight of the lymphoid organs (thymus, Fabricius bursa) of the birds, at both ages. Supplementation in eggs of cut quails with CLA 240 mg reduced the total blood cholesterol content compared to CLA 120 mg, at 21 days of age. At 35 days of age, higher concentrations of CLA and AL increased the total cholesterol and AST in the blood of birds and AL had a greater effect on the increase than CLA.

RESUMO Objetivou-se avaliar a influência da suplementação com minerais biocomplexados sobre a qualidade da carne de frangos da linhagem Label Rouge, de ambos os sexos, criados em sistema alternativo. O delineamento experimental foi inteiramente ao acaso (DIC), disposto em esquema fatorial (3x2), sendo três dietas (controle; 0,50ppm de selênio; 0,40ppm de cromo) e dois sexos. Para a estabilidade lipídica, foi utilizado o DIC, disposto em esquema fatorial (3x2x2), sendo três dietas, dois sexos e dois tempos de armazenamento (zero e 12 meses). Os parâmetros avaliados no peito e na coxa foram: pH final, cor (L* - luminosidade, a* - índice de vermelho, b* - índice de amarelo, C* - índice de saturação e h* - ângulo de tonalidade), perda de peso por cozimento (PPC), força de cisalhamento (FC) e estabilidade lipídica. Além disso, foram avaliadas a proporção das formas químicas da mioglobina da coxa e a quantificação do conteúdo de selênio no peito. Não houve efeito isolado da dieta sobre os parâmetros físicos e químicos do peito e da coxa e sobre a concentração de selênio no peito. As fêmeas apresentaram médias superiores de L* (57,57), b* (10,55) e C* (10,60) do peito; e os machos de L* (55,09) da coxa. Entre os machos, as aves alimentadas com a dieta controle e com cromo expressaram valores superiores de FC da coxa em relação às suplementadas com selênio; para o tratamento com cromo, os machos apresentaram média superior de FC da coxa e, em contrapartida, para o tratamento com selênio, as fêmeas manifestaram a maior média. As amostras submetidas ao período de 12 meses de armazenamento mostraram maiores valores de substâncias reativas ao ácido tiobarbitúrico (TBARS) em ambos os cortes. A suplementação com cromo e selênio biocomplexados não provocou alterações na qualidade da carne; as fêmeas demonstraram melhores atributos de qualidade da carne.

530Usage of probiotic in country-type broiler's chicken diet and its effects over the carcass yield, morphology and the gastrointestinal pH Uso de probiótico em dietas de frangos tipo caipira e seus efeitos sobre o rendimento de carcaça, a morfologia e o pH gastrointestinal

The objective of this study was to evaluatethe effects of including pequi pulp flour (PPF) in laying hendiets onperformance and egg quality.A total of 240 Hisex Brown strain were randomly assigned into 5 groups and were fed diets containing PPF at the level of 0%, 0.125%, 0.250%, 0.375%, and 0.5%. The experiment lasted63 days (3 periods of 21 days).The variables of performance andeggquality were submitted to linear and quadratic regression analysis at the level of 5% probability.Theoutcomes suggest that neither performance nor egg quality wasaffected (p > 0.05) by different levels of PPF.Dietary inclusion ofPPFtested in the present study wasnot sufficient to demonstrate improvement in the productive performance of birdsas well as in egg quality.Because of the lack of responses, additional research is needed to evaluate higher levels of PPF in the diets for laying hens.

Emulsifiers in animal diets are an interesting and effective strategy for nutritionists to improve the digestion of dietary lipids and, consequently, provide better performance. This study aimed to evaluate the effect of the supplementation of diets with emulsifier based on mono- and diglycerides (120 g t-1 of feed) associated with different energy levels (2,775; 2,725; 2,675 and 2,625 kcal of metabolizable energy kg-1 of feed) on performance and egg quality of Hisex Brown laying hens. The laying hens were distributed in 50 experimental plots with eight birds each. A completely randomized design with five treatments and ten replicates/treatments was used. The treatments in this study were: control diet (C) with 2,775 kcal of metabolizable energy (ME) kg-1 of feed; C + E: diet C supplemented with an emulsifier (E) at 120 g t-1 of feed; diet C with reduction of 50 kcal ME and supplemented with E (NC50 + E); diet C with reduction of 100 kcal ME and supplemented with E (NC100 + E); and diet C with reduction of 150 kcal ME and supplemented with E (NC150 + E). All diets had the same inclusion level of degummed soybean oil (2%). There was no influence of the treatments on the performance and quality of the eggs (p >0.05). The current study has shown that diets supplemented with emulsifiers based on mono- and diglycerides (120 g t-1) allows reducing the metabolizable energy level up to 150 kcal kg-1 of feed, without impairment on performance and egg quality of Hisex Brown laying hens

We intended to evaluate the influence of supplementation with biocomplexed minerals on the performance and carcass characteristics of Label Rouge broilers of both sexes, as well as on the feed efficiency and feed costs. The experimental design was completely randomized arranged in factorial scheme (3×2)-three experimental diets (control, 0.50 ppm of selenium, and 0.40 ppm of chromium) and two sexes (male and female). Each treatment was composted of three plots, each one represented by 21 birds. The average weight (g/bird), weight gain (g/bird), feed intake (g/bird), and feed conversion in periods of 1 to 7, 1 to 21, 1 to 35, 1 to 49, 1 to 63, and 1 to 90 days were calculated. Weight and yield of carcass and cuts were determined. Both average feed cost (AFC, R$/bird) and cost per kg of weight gain (CWG, R$/kg weight gain) as well as economic efficiency index (EEI) were estimated. There was no influence of the diet on performance, carcass characteristics, and feed costs of the birds. The males presented higher means of average weight (3819.2 g/bird), weight gain (3780.9 g/bird), feed intake (11030.2 g/bird), and better feed conversion index (2.9) in the period of 1 to 90 days, besides presenting higher values of live weight at slaughter (3740.2 g), carcass weight (3117.5 g) and carcass yield (83.4%), breast weight (832.4 g), thigh weight (432.5 g), and thigh yield (13.9%); the females presented higher breast yield values (28.1%). The males presented higher AFC and lower CWG. The addition of minerals in the diet does not change performance, carcass characteristics and feed costs. The males present better performance and better carcass characteristics, in addition to being economically more viable.

The objective of this study was to evaluate the effects of inoculation in egg with sources of conjugated linoleic acid (CLA) and lauric acid (LA) on hatchability, zootechnical performance and carcass characteristics of quails from one to 35 days of age. On the 7 th day of incubation, the eggs were inoculated with 0.05 mL of the supplement according to the treatments, CLA and LA. After birth, the birds were housed in cages until 35 days. A completely randomized design was used, with six treatments and six replicates of 10 birds per cage. The inoculations in eggs with CLA and LA did not interfere in the performance and carcass characteristics of quails at 21 and 35 days of age. However, may be associated with a decrease in egg hatchability. The highest absolute weight of the gizzard was observed at 35 days, from birds inoculated in egg with CLA 240 mg and LA 60 mg in the incubation phase, in comparison to the other treatments. In conclusion, the reduction in hatchability makes it impossible to recommend the use of conjugated linoleic acid and lauric acid -in ovo.