Abstract. Windyaraini DH, Marsifah T, Mustangin Y, Poerwanto SH. 2019. Detection of transovarial transmission of dengue virus in Aedes spp. (Diptera: Culicidae) from Brontokusuman Village, Yogyakarta, Indonesia. Biodiversitas 20: 2061-2067. Dengue Hemorrhagic Fever (DHF) is an infectious vector-borne disease. It is caused by dengue virus infection through the vector of Aedes spp. Dengue virus can be transmitted transovarially from infected female mosquitoes to their eggs. The presence of transovarial transmission allows maintenance of serotypes of dengue virus in nature. Detection of transovarial transmissions can be used to predict disease outbreaks as an effort to control the dengue virus. Dengue control is generally carried out through control of dengue vectors (mosquitoes) with environmental management, the use of chemical agents (insecticides) and biological control. This study was conducted to determine the existence of transovarial (vertical) transmission, factors affecting it and the resistance status of Aedes spp. against cypermethrin insecticide (pyrethroid) in Brontokusuman Village, Mergangsan Sub-district of Yogyakarta, Indonesia. The study was conducted from September 2018 to February 2019 with larvae sampling in 100 houses. The adult Aedes spp. mosquitoes were used as the sample of immunohistochemical methods and bioassay to get the mosquito resistance status against cypermethrin. The transovarial transmission of dengue virus in Aedes spp. from Brontokusuman Village was found in 3 of 100 samples of Aedes spp. mosquitoes, and the TTI value (Transovarial Transmission Index) was 3%. The transovarial transmission is influenced by the density of the infected vectors, the condition of their eggs and the number of dengue cases. The bioassay test results showed that the resistance status of Aedes spp. can be presumed resistance against cypermethrin insecticide.
Penelitian ini dilakukan untuk mengetahui keanekaragaman Acarina dan faktor lingkungan yang mempengaruhi keberadaan Acarina di Pusat Inovasi Agro Teknologi (PIAT) Mangunan, Yogyakarta. Penelitian ini dilakukan di PIAT Mangunan untuk pengambilan sampel dan Laboratorium Sistematika Hewan bagian Parasitologi Fakultas Biologi UGM untuk preparasi dan identifikasi. Metode yang digunakan adalah ekstraksi sampel menggunakan corong Barlese Tullgren dengan beberapa modifikasi. Sampel diambil pada 3 area vegetasi (sirsak, srikaya, dan sawo) dan setiap area diambil lima titik sampling. Pengukuran lingkungan meliputi kelembaban udara dan suhu udara diukur dengan alat higrometer, pH, dan suhu tanah dengan soil tester digital, serta kelembaban tanah dengan soil tester Takemura DM-5. Acarina yang diperoleh dilihat di bawah mikroskop dan di-mounting dengan larutan hoyer’s. Identifikasi Acarina menggunakan buku identifikasi A Manual of Acarology. Data yang diperoleh dari penelitian dianalisis menggunakan indeks keanekaragaman Shannon-Wiener. Berdasarkan identifikasi Acarina yang telah dilakukan, didapatkan 20 Family dan 28 Genus dan 399 cacah individu. Tingkat keanekaragaman Acarina termasuk dalam kategori sedang. Faktor lingkungan yang mempengaruhi kehadiran Acarina adalah suhu dan kelembaban.THE DIVERSITY OF ACARINA IN AGRO TECHNOLOGY INNOVATION CENTER MANGUNANThis study was aimed at determining the diversity of Acarina and environmental factors that affect the existence Acarina at Agro Technology Innovation Center (ATIC) Mangunan, Yogyakarta. This study was conducting at ATIC Mangunan for sampling and the Animal Systematics Laboratory of the Parasitology, Faculty of Biology, Gajah Mada University for preparation and identification. The method used was sample extraction using Barlese Tullgren funnel with several modifications. Samples were taken at 3 vegetation areas (soursop, sarikaya a.k.a Annona squamosa, and sapodilla) and each area was taken five sampling points. The environmental measurements include air humidity and air temperature measured by means of a hygrometer, pH and soil temperature with digital soil tester, and soil moisture with a soil tester Takemura DM-5. Acarina obtained was seen under a microscope and mounted with Hoyer’s solution. The identification of Acarina using identification book A Manual of Acarology. The data obtained from the study were analyzed using the Shannon-Wiener diversity index. Based on Acarina’s identification, 20 families and 28 genera and 399 individual numbers were obtained. The level of diversity of Acarina is categorized as the medium category. The environmental factors that influence the presence of Acarina are temperature and humidity.
Introduction: Family Culicidae was the presence of a mosquito that had a potential vector to cause the spread of dengue fever and some diseases. Mosquito diversity could be different due to human and environmental factors in those regions. This study aims to identify mosquito (family Culicidae) diversity and characteristics of breeding places as vector control management and mosquito-borne disease prevention in the area of Universitas Gadjah Mada, Yogyakarta. Method: The study was conducted from April to September 2018. A sampling of mosquito larvae and observation of mosquitoes breeding places characteristics were carried out inside and outside the Universitas Gadjah Mada campus building which was divided into 5 clusters, there were Science and Engineering cluster, Medica, Agro, Vocational School, and Social Science. Mosquito diversity in the Universitas Gadjah Mada campus area was analyzed used the Shannon-Wienner diversity index. Result and Discussion: Mosquitoes found in the area of Universitas Gadjah Mada consist of two phases with total number 153 larvae and pupae, namely Aedes aegypti, Aedes albopictus, Anopheles spp., and Culex spp. The mosquito with the highest number of larvae and pupae was Aedes albopictus, while Anopheles spp. Only found 1 pupa phase. The number of obtained containers was 50 containers, with 6 positive containers of mosquito larvae. Mostly, the type of container found with mosquito larvae was bucket. For each container observed, the temperature and pH of water in the container were measured and it was found that the water temperature reached 24 – 28°C with pH 6 – 7. Conclusion: Mosquito diversity from all of the areas in Universitas Gadjah Mada was grouped as a medium category, with the greatest number of mosquito larvae were found in Science and Engineering cluster. Containers were located in the open area had more mosquito larvae. Mosquito control is focused on environmental management, biological control, and chemical use.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.