Detection of transovarial transmission of dengue virus in Aedes spp. (Diptera: Culicidae) from Brontokusuman Village, Yogyakarta, Indonesia

Abstract

Abstract. Windyaraini DH, Marsifah T, Mustangin Y, Poerwanto SH. 2019. Detection of transovarial transmission of dengue virus in Aedes spp. (Diptera: Culicidae) from Brontokusuman Village, Yogyakarta, Indonesia. Biodiversitas 20: 2061-2067. Dengue Hemorrhagic Fever (DHF) is an infectious vector-borne disease. It is caused by dengue virus infection through the vector of Aedes spp. Dengue virus can be transmitted transovarially from infected female mosquitoes to their eggs. The presence of transovarial transmission allows maintenance of serotypes of dengue virus in nature. Detection of transovarial transmissions can be used to predict disease outbreaks as an effort to control the dengue virus. Dengue control is generally carried out through control of dengue vectors (mosquitoes) with environmental management, the use of chemical agents (insecticides) and biological control. This study was conducted to determine the existence of transovarial (vertical) transmission, factors affecting it and the resistance status of Aedes spp. against cypermethrin insecticide (pyrethroid) in Brontokusuman Village, Mergangsan Sub-district of Yogyakarta, Indonesia. The study was conducted from September 2018 to February 2019 with larvae sampling in 100 houses. The adult Aedes spp. mosquitoes were used as the sample of immunohistochemical methods and bioassay to get the mosquito resistance status against cypermethrin. The transovarial transmission of dengue virus in Aedes spp. from Brontokusuman Village was found in 3 of 100 samples of Aedes spp. mosquitoes, and the TTI value (Transovarial Transmission Index) was 3%. The transovarial transmission is influenced by the density of the infected vectors, the condition of their eggs and the number of dengue cases. The bioassay test results showed that the resistance status of Aedes spp. can be presumed resistance against cypermethrin insecticide.

Study of Dengue Virus Transovarial Transmission in Aedes spp. in Ternate City Using Streptavidin-Biotin-Peroxidase Complex Immunohistochemistry

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The practicality of Malaysia dengue outbreak forecasting model as an early warning system

“…It takes 3–8 days before the mosquito become capable of transmitting the virus ( Alto & Bettinardi, 2013 ; Carrington & Simmons, 2014 ) either vertically (transovarial) or horizontally. Vertical or transovarial transmission involves the passage of dengue viruses to the offspring through the eggs of infected female mosquitoes ( Wan Najdah et al., 2021 ; Windyaraini et al., 2019 ; da Costa et al., 2017 ; Joshi et al., 2002 ) while horizontal transmission involves the passage of dengue viruses to a vertebrate host via a vector. Typically, 4 days after being bitten by an infected Aedes mosquito, a person will develop viremia, a condition in which there is a high level of the dengue virus in the blood.…”

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“…Although not yet empirical in Bandar Lampung, several studies have reported transovariums in several cities in Indonesia. Transovary or vertical transmission is the entry of the dengue virus into the mosquito's ovary, so that the eggs that hatch already have the same virus as their parents (Tri Baskoro T. Satoto, Umniyati, Suardipa, & Sintorini, 2013;Tri Baskoro Tunggul Satoto et al, 2014;Wanti, Sila, Irfan, & Sinaga, 2016;Windyaraini, Marsifah, Mustangin, & Soenarwan Hery Poerwanto, 2019).…”