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Staphylococcus (S.) aureus is the main facultative organism of contagious intramammary infections from lactating animals. It is considered a major foodborne organism that can cause food poisoning conditions around the world. Camels are very important to the lifestyle of many countries because they can produce milk that contains the major components such as proteins, energy, vitamins, and minerals. The present study used a polymerase chain reaction (PCR) method on a base of the nuc gene as a target gene, which is a specific gene that recognizes the S. aureus amongst other microorganisms. Fifty milk samples have been collected from camels from different areas of the Nineveh Governorate, Iraq. According to the phenotypic characteristics, isolation and identification of S. aureus have been accomplished by characterizing the shape of the colonies, painting the suspected isolates by gram stain, using the biochemical tests such as coagulase and catalase. In this study, S. aureus was isolated from 70% (35/50) camel milk samples. The classical method of identifying the S. aureus isolated from camel milk was consistent with the PCR method. The PCR technique indicated that all positive S. aureus possessed the nuc gene. The increased percentage of S. aureus isolated from the camel milk has a relationship with the type of farm management, poor nutrition, and/or environmental conditions, rather than treatment of the infected camel. The PCR method is considered one of the best-used techniques to identify the S. aureus isolated from camel milk by detection of nuc gene, the specific gene of S. aureus.

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In general, the Iraqi farmers of breeding and fattening of sheep using a broadspectrum antibiotics like tetracycline and aminoglycosides family for treatment and prophylaxis without considering to the withdrawal periods of these drugs before slaughtering the animals. The aim of the present study was to determine the residues of streptomycin (member of aminoglycosides group) in sheep meat and edible tissues using enzyme linked immunosorbent assay (ELISA) method. A total of 88 samples including longissimus dorsi, diaphragmatic muscles, liver and kidney were collected from 22 sheep carcasses slaughtered in Duhok abattoir, Kurdistan, Iraq. The result revealed that streptomycin residues were found in 12 samples (54.5%) of the sheep carcasses distributed as following 36.36 %, 45.45 %, 22.72 % and 50 % of Longissimus dorsi, diaphragm muscle, liver and kidney samples., respectively. The minimal streptomycin concentration in ovine meat samples was 3.001 ppb and the maximum concentration was 690.307 ppb. The results of this study show that sheep meat, which is sold in Dohuk governorate, often contains the residues of antibiotics due to slaughtered animals might have been treated with veterinary drugs and the proper withdrawal period was not respected before slaughtering.‫ـــــــــــــــــــــــــــــــــــــــــ‬

The aim of this study was to provide field outbreaks data with FAdVs in Ninevah governorate to emphasize the importance of the disease due to high mortality and production losses. A total of 729,500 broilers collected from 64 flocks at 14 different locations in Nineveh governorate during the second half of 2020. were included in this study. Histopathological changes of the liver in infected birds have been studied. Molecular identification of FAdV was accomplished by DNA extraction from liver samples using DNeasy Tissue Kit. Results reviled that there were 51892 mortalities representing 7.11%. It was noted that the broiler flocks were infected during their 2nd-6th weeks of age, being the highest in the 5th week of age. Decreased mortality was detected from July to December. being 11.3, 7.91, 7.08, 6.38, 5.94 and 4.95%, respectively. Microscopical examination of the liver manifested the pathognomonic presence of eosinophilic intranuclear inclusion bodies related to the disease. PCR findings revealed positive results of FAdVs. It could be concluded that the environmental stress and immunosuppressive agents could contribute to the percentage and duration of mortalities in broiler flocks.

The aim of this study was to identify and diagnose of M. haemolytica strains as one of the most important causes of ovine clinical mastitis in Mosul city. One hundred and thirtythree milk samples were directly obtained from the udders of ewes infected by clinical mastitis from November 2020 to January 2021.Standard and conventional methods were followed for isolation and identification of M. haemolytica. Milk samples were cultured on blood agar 7% and MacConkey agar, then it was purified and was stained by Methylene blue. Later, different biochemical tests were conducted. Molecular identification of M. haemolytica depending on 16srRNA gene, followed by sequencing, similarity and phylogenetic tree was generated. The results showed that 62(46.61%) of samples were positive for bacterial isolation, biochemical tests and conventional PCR technique. Sequencing results showed that the positive samples were belonged to M. haemolytica strains. The similarity within strain Ib001 and within strain 39433 were 100%, and 99.47% respectively. Poor management was associated with the high level of mastitis caused by M. haemolytica, so the application of prophylactic programs should be followed to limit the spread of the disease.