A new method was established for simultaneous estimation of Tolperisone and Diclofenac sodium by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Tolperisone and Diclofenac sodium by using Agilent C18 column flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) ACN: phosphate buffer pH 3 (pH was adjusted with orthophosphoricacid), detection wavelength was 240nm.The retention times were found to be 4.645 mins and 2.242 mins. The % purity of Tolperisone and Diclofenac sodium was found to be 100.3% and 99.27% respectively. The analytical method was validated according to ICH guidelines. The precision study was precision, robustness and repeatability. LOD value was 2.17 and 0.0372 and LOQ value was 6.60 and 0.1125 respectively.
A simple reverse phase HPLC method was developed for the simultaneous estimation of Amlodipine and Olmesartan in
bulk and tablet form. Chromatography was performed by isocratic reverse phase separation on a stainless steel column 4.6
x 150mm, symmetry column packed with octa decyl silane bonded to porous silica (C18) with particle size 5 micron with
mobile phase containing TEA Buffer of pH 3.0 and Acetonitrile in proportion of 25:75 respectively. The flow rate was 1.0
ml/ min and effluent was monitored at 258 nm. The retention times were 2.39 min and 3.33 min respectively. The standard
curve was linear over a working range of 05–35 µg/ml for both Amlodipine and Olmesartan and gave an average correlation
coefficient of 0.999, and 0.999 for Amlodipine and Olmesartan respectively. The limit of quantitation (LOQ) of this method
was 2µg/ml for Amlodipine and Olmesartan. The absolute recovery was 100% for Amlodipine and 100.3 for Olmesartan.
Degradation products produced as a result of stress studies did not interfere with the detection of Amlodipine and
Olmesartan and the assay can thus be considered stability-indicating.
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