Three simple and sensitive extractive spectrophotometric methods have been described for the assay of Rasagiline mesylate either in pure form or in pharmaceutical formulations. The developed methods involve formation of coloured chloroform extractable ion-pair complexes of the drug with bromothymol blue (BTB), bromophenol blue (BPB) and bromocresol green (BCG) in acidic medium. The extracted complexes showed absorbance maxima at 414 nm for all three methods. Beer's law is obeyed in the concentration ranges 3.0-30, 3.0.0-30 and 2.0-25µg/ml with BTB, BPB and BCG respectively. The effect of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined for three methods. All the three methods have been validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.
Tenofovir disoproxil fumarate is a nucleotide reverse transcriptase inhibitor that has activity against the hepatitis B and HIV viruses. Three simple and sensitive extractive spectrophotometric methods have been described for the assay of tenofovir disoproxil fumarate either in pure form or in pharmaceutical formulations. The developed methods involve formation of colored chloroform extractable ion-pair complexes of the drugs with triphenylmethane dyes, namely, bromothymol blue (BTB), bromophenol blue (BPB), and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima between 410 and 415 nm. Beer's law is obeyed in the concentration ranges 1.5-25, 1.0-25, and 1.25-25 g mL −1 with BTB, BPB, and BCP, respectively. The effectc of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined. All three methods are validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.
Separation of the kernels:The dried neem seeds are ground slightly with hand and outer shell is removed, kernels are present inside the shell which are separated and made into powder using a Abstract:A simple UV spectrophotometric method for the determination of Azadirachtin is developed exhibiting maximum absorbance using distilled water at 542nm.The present study is based on the detection of sample drug concentration using dichloro methane as diluent. All the conditions required were optimized. Statistical analysis was carried out and results of which were satisfactory. The optical characteristics such as absorption maxima, regression analysis and correlation coefficient are obtained. Recovery studies were close to 100% that indicate the accuracy and precision of proposed method and the non-interference of the formulation excipients. All the valid parameters are summarized.
Five spectrophotometric methods for determination of Amitriptyline HCl have been developed, validated and applied for the assay of the drug in pharmaceuticals. Methods A, B and C are based on ion pair complexation of drug, in acidic buffers, with triphenylmethane dyes viz., Bromothymol blue (BTB), Bromophenol blue (BPB) and Bromocresol purple (BCP). The complexes are extracted into chloroform and absorbance is measured around at 415 nm as function of concentration of the drug. The stoichiometry of the complex is found 1:1 in each case. Method D depends upon charge transfer complexation of neutralized drug with iodine which produces iodide ion whose absorbance at 366 nm is measured as function of concentration of the drug. This complex, too, has 1:1 composition as determined by Job's method. Method E is developed on the basis of oxidation of the drug with alkaline KMnO 4 which generates green colored manganate ion with λmax 610 nm. As the intensity of green color increased with increasing time kinetics of the reaction is followed and calibration curves are constructed by using initial rate and fixed time methods. Excellent recovery studies with high accuracy and precision indicate that the methods can be successfully used in industries for the assay of drug in pure form and pharmaceuticals.
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