SPECTROPHOTOMETRIC DETERMINATION OF AMITRIPTYLINE HCl IN PURE AND PHARMACEUTICAL FORMS

Abstract

Five spectrophotometric methods for determination of Amitriptyline HCl have been developed, validated and applied for the assay of the drug in pharmaceuticals. Methods A, B and C are based on ion pair complexation of drug, in acidic buffers, with triphenylmethane dyes viz., Bromothymol blue (BTB), Bromophenol blue (BPB) and Bromocresol purple (BCP). The complexes are extracted into chloroform and absorbance is measured around at 415 nm as function of concentration of the drug. The stoichiometry of the complex is found 1:1 in each case. Method D depends upon charge transfer complexation of neutralized drug with iodine which produces iodide ion whose absorbance at 366 nm is measured as function of concentration of the drug. This complex, too, has 1:1 composition as determined by Job's method. Method E is developed on the basis of oxidation of the drug with alkaline KMnO 4 which generates green colored manganate ion with λmax 610 nm. As the intensity of green color increased with increasing time kinetics of the reaction is followed and calibration curves are constructed by using initial rate and fixed time methods. Excellent recovery studies with high accuracy and precision indicate that the methods can be successfully used in industries for the assay of drug in pure form and pharmaceuticals.

Chromatographic analysis of bromhexine and oxytetracycline residues in milk as a drug analysis medium with greenness profile appraisal

“…According to a literature review, several analytical methods for the analysis of OTC alone and in combination with other drugs have been reported. These methods include spectrophotometric methods, 10–13 TLC, 14–16 HPLC 17–20 and LC-MS-MS. 21,22 Bromhexine has been determined by spectrophotometric methods, 23–25 TLC, 26,27 HPLC 28–31 and LC-MS-MS. 32,33 After a comprehensive literature review, only one published HPLC method for the analysis of the two drugs in their available veterinary dosage form was identified. 34 To date, no method has been reported for the analysis of the targeted drugs in milk samples.…”

New microextraction methods for the evaluation of bromhexine HCl in pure and pharmacological formulations.

“…BromohexineHCl is a white crystalline powder ,It is also somewhat soluble in chloroform and methylene chloride [1].Bromhexine is a benzyl amine-derived cardiac depressant of vasicine that is generated from the plant Adhatoda vasica [3]. It is an expectorant that reduces the viscosity of the material, making it easier to cough up and dispose [4].The mechanism of action is based on sputum decomposition and dark coughing; Respiratory production helps in the formation of thinner, less thick phlegm [2]. Assisting vasomotor secretion generates a vasomotor secretory effect [5].…”