Simple RP-HPLC method for estimation of diazepam in tablet dosage form

Sruthi, A.; P, Tejaswi.; Thanuja, N.; Kumar, Dhiraj; Sagar, P. Vivek

doi:10.1016/j.jopr.2012.11.029

Cited by 11 publications

(8 citation statements)

References 10 publications

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“…There was no obvious preference for choice of column, except for reversed-phase C 18 [55] or C 8 , [56] which was more prevalent than the alternative phases. Monolithic columns [57], consisting of continuous beds with through pores of organic or inorganic matrix, have found increasing applications for HPLC separations.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 92%

“…A new HPLC polymer stationary phase, MSpak GF column [20], consisting of a highly crosslinked hard gel of polyvinyl alcohol, established rapid and simple chromatographic methods for analyzing benzodiazepines by direct injection of samples (plasma, urine) without the extraction procedure or without the column-switching technique. DAD detection provides an improved means to exclude co-eluting substances [55,58]. Some promising results have been obtained by using electrochemical detectors, but further work is required to see how these detectors compare with the well-established ultraviolet detectors, in terms of overall performance.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

See 1 more Smart Citation

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

Uddin¹,

Victoria²,

Samanidou³

et al. 2014

Pharm Anal Acta

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Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 92%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

Uddin¹,

Victoria²,

Samanidou³

et al. 2014

Pharm Anal Acta

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“…The solution was filtered, suitably diluted and the drug entrapped was determined by RP-C18 HPLC method using a mobile phase consisting of acetonitrile, methanol, and 1% phosphate buffer (pH 3) in a ratio of 18:58:24 (v/v/v) at a flow rate of 1 ml/min and the effluent was monitored using UV detection at 232 nm [17] against drug free beads as a blank.…”

Section: Entrapment Efficiencymentioning

confidence: 99%

Formulation and Evaluation of Diazepam-Loaded Intravaginal Alginate Beads: An Investigation Study for the Treatment of Pelvic Floor Dysfunction

Sabry

2018

Asian J Pharm Clin Res

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Objective: The fundamental objective of this research investigation was to develop intravaginal diazepam (DZ)-loaded alginate beads for the management of acute pelvic floor dysfunction (PFD) pain with minimal sedative effect.Methods: DZ loaded beads were prepared by an ionotropic gelation method using SA (sodium alginate) alone, or in combination with either poloxamer 407 (PL), pectin (PC), or xanthan gum (XG) at different ratios in the presence of different concentrations of calcium chloride as a cross-linking agent. The successfully developed beads were evaluated for the particle size, pH, yield percentage, entrapment efficiency, in vitro bioadhesion, swelling percentage, and in vitro drug release. The stability, ex vivo drug permeation, and sedative action of the optimized beads formulations were studied. Results:The particle size of the formulated beads was from 395±3.3 to 515±2.8 µm, yield percentage was from 68.2±1.7 to 87.5±2.1, entrapment efficiency was from 65.6±1.6 to 87.5±2.1. pH ranged from 6.1±0.2 to 6.8±0.6, bioadhesion strength was from 71.5±1.3 to 87.6±3.1, and swelling percentage was in the range from 53.4±3.1 to 85.2±3.7. Approximately 92.4-72.6% of the loaded dose was released from the prepared beads. The optimized beads showed a good stability under the selected storage conditions. About 74.8%, 71.1%, 68.6%, and 63.4% of the loaded dose permeated through the rabbit vaginal mucosa from F7, F9, F3, and F11, respectively. The formulated beads decreased the sedative action associated with orally or parenterally administered DZ. Conclusion:The developed beads were considered a promising candidate to formulate DZ into a new dosage form for the treatment of PFD with a minimum central nervous system sedation.

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“…A simple HPLC method developed and validated by Sruthi et al with UV detection at 230 nm was used for determination of free diazepam concentration. 17 The reverse experiment with the same protocol was also performed to verify the ability of warfarin sodium and diazepam to displace Sirolimus from its binding site.…”

Section: Determination Of Binding Site Of Sirolimus On Hsa At Ph 74 mentioning

confidence: 99%

Interactions Between Sirolimus and Anti-Inflammatory Drugs: Competitive Binding for Human Serum Albumin

Khodaei¹,

Bolandnazar²,

Valizadeh³

et al. 2016

Adv Pharm Bull

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Simple RP-HPLC method for estimation of diazepam in tablet dosage form

Cited by 11 publications

References 10 publications

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

An Overview on Total Analytical Methods for the Detection of 1,4-Benzodiazepines

Formulation and Evaluation of Diazepam-Loaded Intravaginal Alginate Beads: An Investigation Study for the Treatment of Pelvic Floor Dysfunction

Interactions Between Sirolimus and Anti-Inflammatory Drugs: Competitive Binding for Human Serum Albumin

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