The whole plant of Solanum lyratum THUNB (Solanaceae) is a renowned traditional Chinese medicine. Extracts of this plant and its constituents were reported to exhibit a wide spectrum of biological activities including antitumor, antibacterium, and anti-inflammation.1-3) As part of our search for antitumor compounds, the EtOH extract of S. lyratum was successively partitioned with CHCl 3 , EtOAc, and n-BuOH. The CHCl 3 fraction was sequentially subjected to column chromatography over silica gel, silica gel RP-18, and Sephadex LH-20 to give two new sesquiterpenoids, named lyratol C (1) and lyratol D (2), and two known sesquiterpenoids, dehydrovomifoliol (3) 4) and blumenol A (4). 5) By means of chemical methods and spectroscopic analyses, the structures of the two new compounds were established. In addition, the four isolated compounds were screened for cytotoxicity against three tumor cell lines (HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells), with IC 50 values being in the range 3.7-8.1 mM. Herein we report on the isolation and structure elucidation as well as the evaluation of cytotoxic effects of these four compounds.
Results and DiscussionCompound 1 was obtained as a colorless prismatic crystal. Two new sesquiterpenoids, lyratol C (1) and lyratol D (2), together with two known sesquiterpenoids, dehydrovomifoliol (3) and blumenol A (4), were isolated from the whole plant of Solanum lyratum. Their structures were established by spectroscopic analyses. In vitro, the four compounds showed significant cytotoxic activities against three human cancer cell lines, namely, HONE-1 nasopharyngeal, KB oral epidermoid carcinoma, and HT29 colorectal carcinoma cells, and gave IC 50 values in the range 3.7-8.1 m mM.