A series of tropical maize hybrids, involving 10 single, four double and four three-way crosses were evaluated with their parental inbred lines and three control varieties, at Universiti Putra Malaysia, Serdang, Malaysia, in a randomized complete block design, with three replications. The objectives were to identify hybrids superior for yield, yield components and other agronomic traits, to estimate heterosis revealed by the hybrids, and to estimate broad-sense heritability for important characters in the populations. The hybrids were found to vary highly for grain yield and most other traits measured. High estimates of heterosis were shown by grain yield, ear weight and grain weight per ear, moderate for plant and ear heights, shelling percentage, ear diameter, number of kernel rows per ear, number of kernels per ear row and 100-grain weight. The hybrids flowered and matured earlier than their respective inbred parents. Estimates of broad-sense heritability also varied with characters. Moderate heritability was shown for grain yield indicating a substantial amount of genetic variation in this population of hybrids. Low and negligible heritability for days to silking and 100-grain weight indicate that these traits were very much influenced by environmental factors. Based on the overall performance of the hybrids, the single cross, SC-2, the double cross, DC-11 and the three-way cross, TWC-15, were found to have high potential. No obvious differences were observed in performance among the three categories of hybrids, the single, double and three-way crosses.
Several sources cause skin tissue damage. Kefir probiotics, made from fermented cow's milk, are believed to remove scars during use. Kefir involves a series of immune processes, acute and chronic inflammatory processes, cell division cell migration, chemotaxis, and various differentiations. This research determines the histopathological fibroblast cells in the wound healings in mice (Mus musculus) given 80% kefir probiotic. The method of research was the posttest-only control group experimental design. Mus musculus aged eight weeks with a bodyweight of 18-40 grams were taken in two groups randomly with 32 Mice. The result is that fibroblasts in the negative control group had a smaller mean of 10,25 compared to the mean number of fibroblast cells in the 80% Kefir group, which was 25,18. Thus, it was proven that the effectiveness of Kefir probiotics of wound healing in mice (Mus musculus) was given 80% kefir probiotic (p=0,000 <0.05). There is an effect of kefir probiotic on the healing of cuts in mice based on the calculation of the number of fibroblast cells.
Dadiah is a traditional fermented buffalo milk from West Sumatra, Indonesia. It is one of the healthiest drinks because it contains lactic acid bacteria (LAB), which has many health benefits. Lactic Acid Bacteria (LAB) are a group of bacteria that play a role in the fermentation process of food. Lactic Acid Bacteria (LAB) content in dadiah will affect the quality of dadiah in general.West Sumatra has several dadiah producing areas, one of which is the Sianok area located in the city of Bukittinggi. This research aims to find out the potential of Sianokdadiah as a probiotic food. The method used in this research is a survey method with descriptive analysis. Lactic Acid Bacteria (LAB) contained in dadiah was isolated using de Man RogosaSharge (MRS) media. The isolates were then identified based on their morphology and biochemical properties. The tests carried out include gramme stain, catalase test, fermentative type, retention of gastric juice and bile salts. Furthermore, testing using 16S rRNA molecular identification techniques was conducted to determine the species level. The results of the study obtained a total colony of Lactic Acid Bacteria (LAB) from dadiah 89x109 CFU/g. The identification of the single colony found that the Lactic Acid Bacteria (LAB) obtained was included as Gram-positive, with the type of homofermentative fermentation, catalase negative. It has resistance to stomach acid at pH 3 with a viability of 83.7%, bile salt resistance of 0.3%, and viability of 67.3%. Identification of Lactic Acid Bacteria (LAB) using the 16S sRNA gene, the results of running PCR with a base length of 1428bp. Analysis based on phylogenetic trees showed that Lactic Acid Bacteria (LAB)dadiah from the city of SianokBukittinggi has a relationship with Pediococcusacidilactici. From the results of this research, it can be concluded that dadiah from the city of Sianok Bukittinggi has a good enough potential as a probiotic.
Background: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB) which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra. Design and Methods: A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16S rRNA gene. Results: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zone area of 23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp. Conclusions: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.
The development of a child is very important for parents. Every parent wants their child to grow up well and be a strong and good quality child. One of the efforts that parents can do from an early age is to stimulate with the baby massage method. Massage therapy is one of the complementary therapies that can stimulate tactile-kinesthetic stimulation. Massage therapy given since infancy can increase the stimulation of receptors on the skin, affect brain cells, increase interaction between mother and child, and increase body weight so that children's growth becomes optimal. This community service activity aims to increase community knowledge at the Sinar Gunung VI Posyandu, about baby massage and how to do it, so that babies can grow and develop properly. The method used is lecturing and practicing as well as distributing leaflets on how to do a baby massage that will optimize the growth and develop optimally of children. This activity was attended by 18 mothers who have babies. The results of this activity were increased knowledge and skills of mothers who came to Posyandu Sinar Gunung VI who had babies. The conclusion is that there is an increase in the knowledge and skills of mothers who have infants and higher confidence in practicing complementary therapy for infant massage therapy with mentoring and coaching to optimize the growth and develop optimally of infants.
Stroke is a degenerative disease that often occurs on elderly people. Stroke causes the life quality of elderly people becomes low, in which they will face dependence in various life activities. Fatal and permanent effects due to stroke can be avoided if the patient gets medical services quickly and precisely in 3-5 hours. Usually, this golden period makes stroke quickly overcome and the prognosis is better. FAST is one of stroke early detection methods which is easy to teach and understand for elderly people. FAST stands for Face, Arm, Speech, and Timing. Based on the training and education results, the participants received appreciation from various parties, especially the elderly people in Nagari Jawi-Jawi, Solok Regency, the cadres of the Integrated Services Post, and families with elderlies. Most of the trainees were elderly women aged 61-75 years. The follow-up is a program evaluation that would be carried out by the team of Students Creativity Program in coordination with the local Regional Government to be continued by the cadres of the Integrated Service Post for all the elderly people in Nagari Jawi-Jawi, Solok Regency, and it is also expected that all participants can socialize to their families and surrounding environment about this FAST method.
Ears are one of the most frequently cleaned parts of the body. There are many ways to clean the ears, for example by using cotton buds, using candles and so on. The act of cleaning the ear with cotton buds is not recommended, because it can cause earwax (cerumen prop) to be pushed into the ear so that over time it will cause hearing loss (deafness). Sometimes the use of cotton buds is too deep, causing damage to the eardrum. Education in the form of counseling given to junior high school students aims to reduce the risk of ear damage from an early age due to the use of cotton buds and how to clean the ears properly. Education is held at SMP Yari School Padang. The extension method uses power points and leaflets which are distributed to school students. At the time the counseling was carried out, pretest and posttest were given to see students' knowledge of the material presented. The results of the pretest and posttest showed an increase in students knowledge about the material that had been given with p value=0,000.
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