Poliblend edible coating of suweg starch-chitosan (SSC) was prepared as a package with the addition of essential oil from sweet orange peel as antibacterial because of the high limonene content (95%) to increase the shelf life of malang’s apples. There are 6 variations formulation of the suweg starch: chitosan, namely 10: 0, 9: 1, 8: 2, 7: 3, 6: 4 and 5: 5 in total volume 60 mL. Essential oil of sweet orange peel was added with variations in the concentration of essential oil is 0%; 2%; 4%, and 6%.). Furthermore, characterization was carried out by mechanical testing, Water Vapor Transmission Rate (WVTR), solubility, and swelling of the SSC polyblend edible film and the antibacterial activity test of the SSCO polyblend edible coating on Staphylococcus Aureus bacteria before it was applied on malang’s apples. Organoleptic tests and physical observations of malang’s apples were carried out after the application of edible coating SSCO. The result characterization of the functional groups of polyblend edible film SSCO showed the absorption of the wave number 3373.64 cm−1; 1646.32 cm−1; 1570.12 cm−1; 1416.78 cm−1 and 1337.69 cm−1; and 1040.64 cm−1 with functional groups-OH, N-H, C=C, CH3, and C-O. The variation composition affects on the physical properties of edible film SSC in the mechanical test, WVTR, and WVP with the optimum concentration edible film SSC 7: 3, while the solubility and swelling reach the optimum concentration edible film SSC of 5: 5. The addition of sweet orange peel as essential oil had an effect on the antibacterial activity of polyblend edible film SSCO with a maximum inhibition zone 16.55 mm on concentration 6% for S. aureus bacteria. Based on organoleptic test, consumers prefer edible coating SSC with the lowest essential oil content and malang’s apples can last 22 days on physical performance observations.
Penelitian bertujuan untuk mengetahui kualitas semen cair kambing boer yang disimpan dalam pengencer air kelapa muda dengan penambahan sari kedelai. Penelitian dilaksanakan di Laboratorium Reproduksi Ternak, Fakultas Peternakan, Universitas Brawijaya Malang. Materi penelitian adalah semen kambing boer berusia 3 hingga 4 tahun yang ditampung sebanyak dua kali seminggu menggunakan metode vagina buatan. Metode penelitian adalah eksperimen laboratorium menggunakan rancangan acak kelompok (RAK) dengan empat perlakuan sebagai kelompok dan setiap kelompok terdapat 10 ulangan. Perlakuan penelitian adalah P0 (CEP-3 90% + kuning telur 10% + Putih telur 0,4 %), P1 (Air Kelapa Muda Hijau 80% + Kuning Telur 15% + Sari Kedelai 5%), P2 (Air Kelapa Muda Hijau 80% + Kuning Telur 10% + Sari Kedelai 10%), dan P3 (Air Kelapa Muda Hijau 80% + Kuning Telur 5% + Sari Kedelai 15%). Hasil penelitian dianalisis dengan menggunakan sidik ragam. Hasil penelitian menunjukkan persentase motilitas individu spermatozoa dan viabilitas spermatozoa sampai hari ke-3 penyimpanan antara perlakuan P0 dan P2 tidak memberikan perbedaan yang nyata (P>0,05) tetapi pada P1 dan P3 memberikan perbedaan pengaruh sangat nyata (P<0,01). Persentase abnormalitas pada semua perlakuan tidak terdapat perbedaan nyata (P>0,05) pada hari ke-1 sampai ke-3. Pada P0 dapat mempertahankan kualitas semen cair hingga hari ke-3 dengan nilai motilitas individu 43,00 ± 2,88%, sedangkan P2 dapat menjaga kualitas semen cair hingga hari ke-3 dengan nilai motilitas individu 43,00 ± 1,04%. Simpulannya pengencer air kelapa muda dengan penambahan kuning telur 10% dan sari kedelai 10% dapat menggantikan pengencer Cauda. Epididymal Plasma-3 (CEP 3) sampai hari ke-3 untuk inseminasi buatan.
Background:The conservation of Bali bulls, the Indonesian native breed of cattle, is crucial for cattle breeding in Indonesia. To guarantee the spread of Bali bulls through artificial insemination the quality of the frozen semen must be high. To this end, using an extender material in addition to semen increasing spermatozoa's survival during cryopreservation is important. Green tea extract (GTE) can be used as cryoprotectant because its high antioxidant activity can help avoid reactive oxygen species formation.Methods: Semen of five Bali bulls from the National Artificial Insemination Center at Singosari, Indonesia was collected routinely twice a week. First, fresh semen inspection was performed to determine the feasibility of using Bali bulls as animal samples. The extender used in this study was Tris-based egg yolk. The divided samples into four treatments: T0 (no GTE added to the extender), T1 (0.05 mg GTE plus 100 mL extender), T2 (0.10 mg GTE plus 100 mL extender), and T3 (0.15 mg GTE plus 100 mL extender). The semen freezing process was conducted according to standard procedures and sperm quality parameters, i.e., sperm motility, viability, abnormalities, and membrane integrity observed pre-freezing and postthawing.Results: There were significant differences in total motility, progressive motility, viability, and integrity membrane of Bali bull sperm at both pre-freezing and post-thawing after adding GTE into the extender. In contrast, there were no differences in abnormalities among treatments. Conclusion:Adding GTE at a 0.15 mg into 100 mL Tris-based egg yolk extender can improve the quality of cryopreserved Bali bull sperm.
The purpose of this study was to know the difference of liquid semen quality Ongole crossbred in CEP-3 (Bovine Serum Albumin (BSA) in CEP-2 substituted with 0,4% albumen) and tris aminomethane yolks without raffinose which stored in thermos contained with ice cubes. Research was conducted at Sumber Sekar Laboratory Animal Husbandry Faculty of Brawijaya University. The variables observed were motility, sperm membrane integrity and total motile sperm. Data were analyzed using unpaired design T test, total motile sperm tested using Pearson's Chi Square with expectation value of 40 million motile sperm/ml sperm. The results showed that the extenders treatment of P1 and P2 did not give significant difference (p> 0,05) to sperm motility, but gave significant difference (p <0,05) on the 4 th and 6 th hours preservation to sperm membrane integrity of Ongole crossbred stored in ice filled thermos. The conclusion of the research was CEP-3 and tris aminomethan yolks without raffinose have the same ability to maintenance quality of Ongole crossbred's liquid semen stored in ice filled thermos.
Sonok cattle are a culture that still exists on the island of Madura, this culture competes for a pair of female cows to walk with a compact step. Sonok cattle breeders are spread over four districts. The problems faced by Sonok cattle breeders in Waru Barat Village are related to genealogies and profiles that have never been documented, this causes detailed information related to pedigrees and profiles to be unclear. In addition, the marketing process is still carried out with limited media so that a lot of information is not conveyed, such as genealogical details and profiles. One of the villages that is a partner and the majority of its residents are sonok cattle breeders is Waru Barat Village, Waru District, Pamekasan Regency. The service method is a survey that takes samples from a certain time from a population and uses basic data interview techniques. After that, a trial was carried out and some analysis of the application of pedigree, profile, and market applications on Sonok cattle activists, it can be seen that sonok cattle culture activists are greatly facilitated by all the features in the application, pedigree and profile data can be seen through the application so that during sales transactions they can be assured. buyer. From the results of the calculation of the System Usability Scale (SUS) questionnaire filled in by the sonok cow culture activists, the average value is 82. The age of the breeder is 51-60 years (40%) with livestock experience 21-30 years (30%) and the number of maintenance is 2 tail (40%) and ST level 2.25-3 (60%). The results of the analysis show a very good assessment of SUS for Sonok cattle breeders
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