A module for laboratory practice in quantitative analytical chemistry subject has been developed. The method uses digital image colorimetry as a basis for low-cost spectrophotometry analysis. Reduction-oxidation reaction of silver nanoparticle and mercury (II) ion was used as a case study for the colorimetric experiment. The yellow color of silver nanoparticle was faded linearly to colorless with the addition of mercury ion as an analyte. The color change was then recorded with a digital camera and processed through computer software to extract the color information in the form of Red, Green and Blue. The module of laboratory practice was then applied to students in chemical education study program. The gain of the learning process was measured using pre-test and post-test. The results of the study indicate that the students were able to understand the concept of spectrophotometry by using the developed module of digital image colorimetry. Therefore, we conclude that laboratory practice of digital image colorimetry is applicable as an alternative to the traditional spectrophotometry.
This study aimed to investigate the effectiveness of leaf ethanolic extract of Etlingera hemisphaerica (LE3H) in reducing defects in fetal anatomy and endochondral ossification in mice induced by HgCl2 during the post-implantation period. Pregnant mice were divided into four groups, each consisting of 10 dams, and received drink and food ad libitum. The first group was administered LE3H (E1), the second one HgCl2 (E2), the third one HgCl2+LE3H (E3), and the fourth was control (E0), administered double-distilled water only. HgCl2 (5 mg/kg bw) was administrated by injection intraperitoneally on gestation day (GD)9 and LE3H (0.39 mg/g bw) was administered by gavage on GD10. The treated and control animals were killed by cervical dislocation on GD18, dissected, and the morphologically normal living fetuses (MNLF) were collected. The MNLF of E0, E1, E2, and E3 from 5 dams were fixed with Bouin solution, and observed using the free hand razor blade technique for soft tissue examination. The remaining MNLF were fixed with 96% ethanol, and then stained with Alizarin Red S and Alcian Blue for ossification examination. Index of length of ossified part (ILOP) of humerus, index of width of ossified part (IWOP) of humerus, ILOP of femur, and IWOP of femur were calculated. E2 had higher cases of anatomical defects (74,6%) than E3 (48.9%), E1 (15.0%), and E0 (0%). E2 had humerus IWOP of 0.82±0.03, which was significantly lower than that of E0 (0.89±0.04) and E1 (0.89±0.03), while that of E1 and E0 was not significantly different from each other. Meanwhile, IWOP in E3 (0.88±0.03) was significantly higher than that in E2, but not different from that in E1 and E0. Thus, LE3H mitigated defects in fetal anatomy and endochondral ossification induced by HgCl2 in mice.
This article describes the study of behavior on H. spinosa in ex-situ conservation areas at UNIB. Methods: The study used four individuals (1 female and 3 males) who were available in the conservation area. Research duration in 12 days (March-April 2021) with checking periods in the morning, afternoon, evening and night. Results: Observational data found 5 types of behavior, namely; Basking, Bathing, Sleeping, Resting, and Eating, Sexual behavior was not found. Conclusion: The behavior of H.spinosa shows a tendency of low mobility.
This study aimed to measure the effect of red onion extract (Allium cepa) and green bean sprouts (Vigna radiata) on the growth of kebiul plants through cuttings with a comparison of synthetic growth regulators (Rootone-F) and control (without treatment). The research method used RAL (4 treatments, 12 replications) with an observation time of 6 weeks (12 observations) and measurements were made in the afternoon. Observation parameters were the number of seedlings growing, the number of shoots, time of shoot growth, shoot growth rate and the number of leaves. The results showed that the treatment with onion extract and mung bean sprouts better responded to the number of seedlings grown, number of shoots, shoot growth time, shoot growth rate and number of leaves compared to Rootone-F treatment and control. In conclusion, the administration of onion extract and mung bean sprouts could accelerate the growth of kebiul cuttings.
Keywords: Shallots, Kebiul, Cuttings, Green Bean Sprouts
This study aimed to measure the effect of Bambusa vulgaris var and Musa paradisiaca extract on Kebiul (Caesalpinia sp) cuttings were compared with synthetic PGR (Rootone-F) and controls. This research was conducted for six weeks (March-April 2021). Kebiul is a traditional medicinal plant that used by Bengkulunese but mostly they only harvest from nature without cultivating the plant. Bamboo shoots (B. vulgaris var) and banana hump (M. paradisaca) extract are natural PGRs that are commonly used. The content of gibberellins in bamboo plays a role in breaking dormancy, while cytokinins in bananas play a role in increasing cell growth and development. The results showed that the treatment of B.vulgaris var and M.paradisaca extracts gave a good response to the number of growing , number of shoots, growth time, growth rate, and number of leaves. The giving of extracts of B.vulgaris var and M. paradisaca was able to accelerate the growth of kebiul cuttings.
This study aims to describe the activities of lecturers, student activities and student learning outcomes on creative thinking material in biology study programs, by applying the Problem-Based Learning (PBL) learning model. This type of research is classroom action research (CAR) with descriptive methods. This research was conducted in 2 cycles, each cycle consisting of 4 stages: planning, implementing, observing and reflecting. Research subjects are lecturers and all semester 5th students of biology study programs. The research variable is the learning model Problem-Based Learning and student learning outcomes. Data collection techniques used are observation and tests. While the research instruments consisted of observation sheets and test sheets. The results of the analysis of lecturer activity data in cycle 1 obtained an average of 33.5 (good) and cycle 2 rose to 35 (good). The results of the analysis of student learning activity data cycle 1 obtained an average score of 29 (good) cycle 2 increased to 34 (good). Student learning outcomes tested by the T-test showed that significantly showed an increase in the value of learning outcomes in cycle 1 (3.02439) and in cycle 2 (7.26829). It was concluded that the Problem-Based Learning (PBL) learning model can improve student learning activities as well as student learning outcomes that take entrepreneurship courses in Biology education courses.
This study aims at investigating whether the implementation of “course system horay” is able to boost the students’ outcome and their interest in the subject of general biology, the odd semester of the 2019/2020 academic year. The type of this study is categorized as classroom action research with 50 students as the subject research. The data collection was conducted using observation, questionnaires, paper-based tests, and documentation. Meanwhile, the technique of analysis was done using descriptive study. By implementing the course system horay, we report that there is a significant increase in the students’ outcome in the general biology course. The analysis also reveals that the mean scores of students’ interest are higher on average.
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