18Primary cilia are a unique organelle, known to provide a signaling hub for variety of cell 19 activities. Their potential role(s) in human immune homeostasis and diseases, however, have 20 yet to be explored. Here, we show that human dendritic cells (DCs) express primary cilia-like 21 structure. The primary cilia growth during DC proliferation by GM-CSF was shut off by DC 22 maturation agents, suggesting the role of primary cilia to transduce proliferation signaling. 23PDGFRα pathway, one of proliferation signal in primary cilia, promoted DC proliferation in a 24 dependent manner of intra-flagellar transport system. In epidermis with atopic dermatitis 25 patients, aberrant ciliated langerhans cells and keratinocytes with showing immature state 26 were observed that may play a potential role in inflammation and skin barrier disorder. 27
Primary cilia influence cell activity, and thus have a unique role in maintaining cell proliferation and differentiation. In atopic dermatitis (AD) and psoriasis, areas of skin inflammation exhibit dysregulated keratinocyte homeostasis. The role of primary cilia in these conditions remains unclear. The objectives of this study is to elucidate the incidence of primary cilia in skin inflammation and the potential mechanism underlying the dysregulation of keratinocytes. Primary cilia were observed using immunofluorescence staining. Normal skin samples were compared with skin samples from patients with AD or psoriasis in terms of cilia numbers and length. The effect of cytokine stimulation on ciliogenesis in keratinocytes was analysed using a primary keratinocyte culture. IFT88, an important ciliary intraflagellar protein, was blocked in Th2 and Th17 cytokines‐stimulated keratinocytes. These effects were analysed with quantitative polymerase chain reaction and Western blot. Significant increases in ciliated cells were observed in AD and psoriasis skin samples compared with normal skin samples. The stimulation of keratinocytes using Th2 and Th17 cytokines modulated the formation of primary cilia. The amount of IFT88 in the primary cilia associated with the phosphorylation of JNK, but not p38, in keratinocytes stimulated with interleukin‐13, 17A and 22. An increase of ciliated cells in the epidermis may impair keratinocyte differentiation under stress conditions caused by inflammation in both AD and psoriasis patients.
Palm Fatty Acid Distillate (PFAD), a by-product of CPO industry, contains approx. 82%-wt of free fatty acids, which can be utilized as raw material for Magnesium Salts of Fatty Acids (Mg-PFAD). The objectives of the experiment were to produce Mg-PFAD salts through saponification-fusion reaction of PFAD at low temperature and ambient pressure and investigate the effect of MgO to fatty acid molar ratio on reaction conversion and yield. Next, washing Mg-PFAD salts with ethanol was able to facilitate the recovery of vitamin E (tocopherol and tocotrienol) from PFAD. Composition of Mg-PFAD were determined by AAS and GC analysis. Based on the data, yield of Mg-PFAD was increased by the increased of MgO to PFAD molar ratio. Mg-content of the product was within the standard according to FAO reference (4-5%-wt). Reaction was completed within 5-7 min after the addition of H2O (as catalyst).
Introduction: Mangosteen (Garcinia mangostana L.), is an evergreen of the Guttiferae family that carries antioxidant activity. Objectives: to examine the antioxidant activity of the leaves, branches and rinds of the mangosteen using DPPH and CUPRAC methods, total phenolic content (TPC) and total flavonoid content (TFC), analyze the correlation between TPC, TFC and antioxidant activity, the correlation between two methods, and found the levels of flavonoid compounds. Methods: Extraction was performed by reflux method using solvents with graded polarity, namely n-hexane, ethyl acetate and ethanol. Determination of antioxidant activity with DPPH and CUPRAC, TPC and TFC were performed by UV-visible spectrophotometer. The correlation between TPC, TFC and antioxidant activity of DPPH and CUPRAC as well as the correlation between two methods were conducted by Pearson's method. The level of flavonoid compounds was performed by HPLC. Results: Mangosteen leaves, branches and rinds extracts had antioxidant activity of DPPH in the range of 39.920 -489.708 mg AAE/g and antioxidant activity of CUPRAC in the range of 116.360 -570.400 mg AAE/g. The highest TPC was given by the ethanol leaves extract (49.525 ± 4.263 g GAE/100 g) and the highest TFC was given by the n-hexane rinds extract (13.859 ± 1.451 g QE/100 g). The ethanol rinds extract contained rutin 0.0327% and kaempferol 0.0049%. Conclusions: TPC and TFC correlated positive and significant with the value of antioxidant activity, except for the n-hexane leaves extract using the DPPH method. The DPPH and CUPRAC methods gave linear results in determining the antioxidant activity of mangosteen extracts.
Introduction: Atopic dermatitis (AD) is a common allergic eczema that affects up to 10% of adults in developed countries. Immune cells in the epidermis, namely, Langerhans cells (LCs), contribute to the pathogenesis of AD, although their exact role(s) in disease remain unclear.Methods: We performed immunostaining on human skin and peripheral blood mononuclear cells (PBMCs) and visualized primary cilium.Result and discussion: We show that human dendritic cells (DCs) and LCs have a previously unknown primary cilium-like structure. The primary cilium was assembled during DC proliferation in response to the Th2 cytokine GM-CSF, and its formation was halted by DC maturation agents. This suggests that the role of primary cilium is to transduce proliferation signaling. The platelet-derived growth factor receptor alpha (PDGFRα) pathway, which is known for transducing proliferation signals in the primary cilium, promoted DC proliferation in a manner dependent on the intraflagellar transport (IFT) system. We also examined the epidermal samples from AD patients, and observed aberrantly ciliated LCs and keratinocytes in immature and proliferating states. Our results identify a potential relationship between the primary cilium and allergic skin barrier disorders, and suggest that targeting the primary cilium may contribute to treating AD.
Objectives: The goals of this research were to observe antioxidant properties from different parts of Simana lagi apple ([Malus domestica Borkh.] "Simana lagi") using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50 (IC 50 ) of DPPH and IC 50 of ABTS.Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and ABTS assays, determination of total phenolic, and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC 50 of DPPH and IC 50 of ABTS activities were analyzed by Pearson's method. Results:The ethanolic leaves extract (LV3) of Simana lagi apple also exposed the highest total phenolic content (TPC) (13.88 g gallic acid equivalents/100 g), while the highest total flavonoid content was presented by ethyl acetate extract (LV2) (7.21 g QE/100 g). The lowest IC 50 of DPPH scavenging activity 0.19 µg/ml, and the lowest IC 50 of ABTS scavenging activity 0.15 µg/ml was given by ethanolic flesh extract (FL3) of Simana lagi apple. There were significantly negative correlation between TPC in all different parts extracts of Simana lagi apple with their IC 50 of DPPH and IC 50 of ABTS. Conclusions:All different parts extracts of Simana lagi apple were categorized as very strong antioxidant by DPPH and ABTS method (except n-hexane peels extract and n-hexane LV by DPPH method, and n-hexane FE by ABTS method). The major contributor in antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple by DPPH and ABTS methods were phenolic compounds. Antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple showed linear results by DPPH and ABTS methods.
BACKGROUND: Many vegetables and fruits have been shown to be sources of antioxidant such as lemons, apples, cabbage, mangoes, beets, and guavas AIM: This research aimed to determine antioxidant activity of Cucumis sativus L. (cucumber) pulp and leaves extracts using DPPH and CUPRAC methods, total phenolic content (TPC), total flavonoid content (TFC), correlation of TPC and TFC on antioxidant activity, correlation between the two methods, identification of marker, and total marker content. METHODS: Antioxidant activity was examined by determining IC50 and AAI of DPPH and EC50 and AAI of CUPRAC. TFC and TPC was measured using UV-visible spectrophotometer. Correlation of TPC and TFC on antioxidant activity was analysed by Pearson’s method. RESULTS: AAI of DPPH of cucumber pulp and leaves extracts in the range of 0.22 - 2.18, whereas AAI of CUPRAC 0.07 - 0.95. All extracts showed antioxidant activity. Ethyl acetate cucumber pulp extract had highest antioxidant by DPPH assay, whereas n-hexane cucumber leaves extract had highest antioxidant activity by CUPRAC assay. Ethyl acetate cucumber leaves extract had highest TFC value (21.47 g QE/100 g) and TPC value (2.34 g GAE/100 g). Flavonoids in cucumber pulp extract contributed to antioxidant activity of CUPRAC method and phenolic compounds in cucumber pulp extract gave a contribution to antioxidant activity of DPPH method. Quercetin content as marker in ethanol cucumber pulp extract was 0.00114%. AAI CUPRAC and DPPH of cucumber leaves extract showed positive correlation but not significant. CONCLUSION: Antioxidant activity between CUPRAC and DPPH methods on cucumber extracts were not linear.
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