Phytochemical and Antioxidant Profile: Cucumber Pulp and Leaves Extracts

Insanu, Muhamad; Zahra, Aliya Azkia; Sabila, Nurma; Silviani, Velina; Haniffadli, Ariranur; Rizaldy, Defri; Fidrianny, Irda

doi:10.3889/oamjms.2022.8337

Open Access Maced J Med Sci

2022

DOI: 10.3889/oamjms.2022.8337

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Phytochemical and Antioxidant Profile: Cucumber Pulp and Leaves Extracts

Muhamad Insanu¹,

Aliya Azkia Zahra²,

Nurma Sabila

et al.

Abstract: BACKGROUND: Many vegetables and fruits have been shown to be sources of antioxidant such as lemons, apples, cabbage, mangoes, beets, and guavas AIM: This research aimed to determine antioxidant activity of Cucumis sativus L. (cucumber) pulp and leaves extracts using DPPH and CUPRAC methods, total phenolic content (TPC), total flavonoid content (TFC), correlation of TPC and TFC on antioxidant activity, correlation between the two methods, identification of marker, and total marker content. … Show more

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Cited by 4 publications

References 21 publications

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Acylated Flavonoids from Cucumis sativus Inhibit the Activity of Human Pancreatic Lipase

Olennikov,

Kashchenko

2023

Appl Biochem Microbiol

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Lipid metabolism disorders are a large group of diseases for the treatment of which various strategies are used, including the use of pancreatic lipase inhibitors that reduce the intake and adsorption of lipids. This study was the first that shows that agricultural wastes of cucumber (Cucumis sativus) can be a source of the effective lipase inhibitors. As a result of the chromatographic separation of metabolites in C. sativus leaves, seven acylated flavonoids were identified, including three new derivatives of isovitexin characterized by UV, NMR spectroscopy and mass spectrometry data as isovitexin-2"-O-glucoside-6"-O-ferulate (1), isovitexin-2"-O-glucoside-6"-O-p-coumarate (2), and isovitexin-2"-O-(6"-O-feruloyl)-glucoside-6"-O-ferulate (3). The quantitative HPLC data showed that the total content of the acylated flavonoids in the leaves of Russian varieties of C. sativus amounted to 3.78–7.44 mg/g of dry plant weight. Isolated compounds demonstrated the ability to inhibit the human pancreatic lipase; the effectiveness of compound 3 was the greatest and exceeded the activity of the reference compound Orlistat. This study has shown that C. sativus leaves can be a useful source of biologically active phytocomponents with hypolipidemic activity.

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Acylated Flavonoids from Cucumis sativus Inhibit the Activity of Human Pancreatic Lipase

Olennikov,

Kashchenko

2023

Appl Biochem Microbiol

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Acylated Flavonoids from <i>Cucumis sativus</i> Inhibit Activity of Human Pancreatic Lipase

Olennikov,

Kashchenko

2023

Prikladnaâ biohimiâ i mikrobiologiâ

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Lipid metabolism disorders are a large group of diseases for the treatment of which various strategies are used including the use of pancreatic lipase inhibitors reduced the intake and adsorption of lipids. This study was the first shown that agricultural wastes of cucumber (Cucumis sativus) can be a source of the effective lipase inhibitors. As a result of the chromatographic separation of C. sativus leaves metabolites, seven acylated flavonoids were identified, including three new derivatives of isovitexin characterized by UV, NMR spectroscopy and mass spectrometry data as isovitexin-2"-O-glucoside-6"'-O-ferulate (1), isovitexin-2"-O-glucoside-6"'-O-p-coumarate (2), and isovitexin-2"-O-(6"'-O-feruloyl)-glucoside-6"'-O-ferulate (3). The quantitative HPLC data showed that the total content of the acylated flavonoids in the leaves of Russian varieties C. sativus amounted to 3.78–7.44 mg/g of dry plant weight. Isolated compounds demonstrated the ability to inhibit the human pancreatic lipase and the effectiveness of compound 3 was the greatest and exceeded the activity of the reference compound Orlistat. This study has shown that C. sativus leaves can be the useful source of biologically active phytocomponents with hypolipidemic activity.

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Green Waste from Cucumber (Cucumis sativus L.) Cultivation as a Source of Bioactive Flavonoids with Hypolipidemic Potential

Olennikov,

Kashchenko

2023

Agronomy

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Cucumber is an important agricultural crop that is cultivated under greenhouse conditions. Cucumber cultivation generates substantial quantities of green waste that require proper disposal. The lack of data on the chemical composition of cucumber greens and their biological activity emphasizes the necessity for research on useful products resulting from this widely available waste. Our examination of the flavonoid contents in the leaves and stems of 30 cucumber cultivars revealed 6 cultivars with high flavonoid contents. In addition, the cutting time did not cause negative effects, and we observed a positive effect with 0.05–0.10% organomineral fertilizer application on the flavonoid levels in the greens. Liquid chromatography–mass spectrometry detected 38 apigenin derivatives, including acylated and non-acylated cucumerins and C-, O-, and C,O-glycosides. Among these, 12 known flavonoids and 18 novel compounds were identified. The concentrations of these compounds in the six flavonoid extracts varied at 39.85–181.53 mg/g for the non-acylated flavones, 14.67–293.31 mg/g for the cucumerins, and 401.73–892.17 mg/g for the acylated flavones. Oral administration of the total flavonoid extracts (at a dosage of 100 g/kg/day) resulted in a hypolipidemic effect in hyperlipidemic hamsters, with subsequent normalization of their serum lipid profiles, malondialdehyde levels, and liver antioxidative enzyme activities. These results substantiate the lipid-lowering potential of cucumber waste extracts. Our investigation of the selected flavonoid activity showed that isovitexin-2′′-O-glucoside-6′′-O-p-coumarate (administered at 50 g/kg/day) had the highest hypolipidemic potency. These results can contribute to the practical use of cucumber green waste and the development of novel supplements for diseases linked to high-fat consumption.

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Phytochemical and Antioxidant Profile: Cucumber Pulp and Leaves Extracts

Cited by 4 publications

References 21 publications

Acylated Flavonoids from Cucumis sativus Inhibit the Activity of Human Pancreatic Lipase

Acylated Flavonoids from Cucumis sativus Inhibit the Activity of Human Pancreatic Lipase

Acylated Flavonoids from <i>Cucumis sativus</i> Inhibit Activity of Human Pancreatic Lipase

Green Waste from Cucumber (Cucumis sativus L.) Cultivation as a Source of Bioactive Flavonoids with Hypolipidemic Potential

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