The in vitro effect of four isolates of the nematophagous fungi Duddingtonia flagrans (AC 001), Monacrosporium sinense (SF 53) and Pochonia chlamydosporia (VC 1 and VC 4) on eggs of Schistosoma mansoni was examined. One thousand S. mansoni eggs were plated on 2% water-agar with the grown isolates and control without fungus. After 7, 14 and 21 days, the eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, besides hyphal penetration and internal egg colonization. Significant differences (P \ 0.01) were found among the studied fungal isolates for ovicidal activity, confirming type 3 effect for the isolates VC 1 and VC 4, which characterizes the ovicidal activity of a fungus. Type 3 effect was only found for P. chlamydosporia (VC 1 and VC 4) with 26.6 and 17.2%, 25.6 and 22.6%, 27.4 and 23.9% in the 7, 14 and 21 days respectively (P \ 0.01). P. chlamydosporia can thus be a potential biological control agent for S. mansoni eggs.
Moluscos são hospedeiros intermediários de Angiotrongylus vasorum, parasito com potencial zoonótico que tem os canídeos como hospedeiro definitivo. Neste trabalho, foram seccionados os tecidos de 180 espécimes de Achatina fulica infectados com 1.000 L1 de A. vasorum, os quais foram submetidos às seguintes técnicas: hidróxido de potássio 1% (grupo I), ácido clorídrico 3% (grupo II), ácido clorídrico e pepsina 1% (grupo III) em banho-maria a 37 °C e Baermann a 42 °C (grupo IV). No primeiro, oitavo e trigésimo dias após infecção (dpi), foram obtidos L1, L2 e L3, e 240 L3 recuperadas do grupo III foram fornecidas a um cão. Os resultados mostraram no grupo I: 59% L1, 7% L2 e 13% L3; no grupo II: 31% L1, 13% L2 e 27% L3, no grupo III: 23% L1, 22% L2 e 30% L3 e no grupo IV: 0,3% L1, 0,02% L2 e 5% L3. Observaram-se larvas vivas no grupo III: 15,5% L1 e 8,22% L3; no grupo II: 9,8% L3 e no grupo IV: 100% L1, L2 e L3. No 56° dpi, encontrou-se L1 nas fezes do cão. Pelas técnicas avaliadas, foram recuperados e identificados todos os estádios de A. vasorum intramolusco, confirmando o potencial de A. fulica como hospedeiro intermediário do nematoide.
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