<p>Tanaman Kesembukan (Paederia foetida L.) merupakan salah satu tanaman dari suku Rubiaceae (kopi-kopian) yang telah digunakan oleh masyarakat sebagai obat sariawan, obat demam, sesak nafas, obat tekanan darah tinggi, rematik dan herpes. Tujuan dari penelitian ini adalah untuk mengetahui tingkat toksisitas masing-masing ekstrak tanaman Kesembukan terhadap larva udang Artemia salina L. dan untuk mengetahui kandungan senyawa yang terkandung dalam masing-masing ekstrak tanaman kesembukan.</p><p>Penelitian ini dilakukan dengan mengekstraksi sampel menggunakan pelarut etanol, kloroform dan n-heksana. Ekstrak pekat yang diperoleh digunakan untuk uji toksisitas terhadap larva udang Artemia salina L. dengan metode Brine Shrimp Lethality Test (Meyer, et al., 1982) dan untuk uji fitokimia dengan reagen. Senyawa dari hasil uji fitokimia diidentifikasi menggunakan kromatografi lapis tipis dengan beberapa eluen untuk mengetahui eluen yang terbaik. Data kematian larva udang Artemia salina L. dianalisis dengan analisis probit untuk mengetahui nilai LC<sub>50</sub> pada masing-masing ekstrak.</p>Hasil dari penelitian ini menunjukkan bahwa masing-masing ekstrak tanaman Kesembukan (Paederia foetida L.) memiliki tingkat toksisitas terhadap larva udang Artemia salina L.yang ditunjukkan dengan nilai LC<sub>50</sub> kurang dari 1000 ppm. Nilai LC<sub>50 </sub>masing-masing ekstrak etanol, kloroform dan n-heksana adalah 226,787; 482,014; dan 146,181 ppm. Kandungan senyawa hasil uji fitokimia menggunakan reagen untuk masing-masing ekstrak tanaman Kesembukan adalah golongan senyawa steroid. Eluen terbaik untuk pemisahan senyawa pada ekstrak etanol adalah n-heksana : etil asetat (8:2) dengan nilai Rf= 0,22 dan 0,48. Pada ekstrak kloroform adalah n-heksana : etil asetat (7:3) dengan nilai Rf= 0,32; 0,45; 0,59; 0,63; 0,82; 0,85; 0,87 dan 0,94. Pada ekstrak n-heksana adalah n-heksana : etil asetat (8:2) dengan nilai Rf= 0,12; 0,38 dan 0,41.
Waru jawa (Hibiscus tiliaceus L.) is a plant that functions as an anti-inflammatory. Compounds from the waru jawa plant, especially the bark can be grouped into alkaloids, flavonoids, triterpenoids, and steroids. The content of triterpenoids from the waru jawa stem bark was tested for their biochemical activity, so that it is expected that the bark of Javanese waru stems can be used optimally. This study aims to determine the potential of triterpenoids from waru jawa stem bark as anti-inflammatory candidates in mice (Mus musculus) rheumatoid arthritis model in silico-based. Potential analysis of triterpenoids in mice was carried out using the STITCH, which is a database of known and predicted interactions between chemicals and proteins found in living things. Interactions in question are physical and functional associations, the data contained in STITCH comes from computational predictions, transfer of knowledge between organisms, and from interactions collected from other databases. Analysis of the triterpenoid mechanism of waru jawa stem bark against rheumatoid arthritis model mice in silico-based using the bioinformatics application Kegg. Based on the results of the analysis, it can be seen that triterpenoids derived from waru jawa stem bark sources play an important role in reducing inflammation. The triterpenoids target NF-κB, leading to its downregulation. Triterpenoids have been found to have many functions, although their effective concentrations for various cellular effects may vary widely. Depending upon the dose administered, triterpenoids can induce anti-inflammatory, proliferation-arresting, apoptotic effects, cytoprotective, and tumor-differentiating.
An amperometric biosensor for glucose determination was developed using a carbon paste electrode (CPE) modified with cellulose acetate (CA)/glucose oxidase (GOx) bilayer membrane through the electrostatic interaction between them. The CA membrane was used as matrix for enzyme immobilization via microencapsulation technique, is enzyme placed between two membranes. CA/GOx membrane was attached to CPE surface containing ferrocene (Fc). By using proposed amperometric biosensor, glucose concentration was determined as well as its characteristic. The modified Fc–graphite electrode with CA/GOx bilayer membrane for glucose had optimum measurement conditions at work emf of 874 mV, CA concentration of 10% and amount Fc of 0.021 mg. The biosensor showed good performance at glucose concentration range of 0.05–3 mM and limit of detection was 0.024 mM. Proposed biosensor has good reproducibility with relative standard deviation (RSD) was less than 5% up to 7 times use (in the defined condition 4 ˚C). Glucose measurement result in human serum of diabetes mellitus patients showed conformity with result of reference method, MediSense Optium glucose test kit.
Daun Calathea Silver merupakan tanaman hias yang memiliki warna dasar ungu di bagian bawah dan warna perak di atas daunnya. Pada dasarnya jika tanaman memiliki warna ungu, maka kemungkinan senyawa metabolit sekunder yang terkandung di dalamnya mayoritas adalah senyawa flavanoid dan memiliki aktivitas antioksidan yang tinggi. Penelitian ini bertujuan sebagai skrinning awal dalam menentukan kandungan senyawa metabolit sekunder yang ada di dalam ekstrak etanol daun Calathea silver berdasarkan uji reagen fitokimia dan profil persebaran senyawa kimia menggunakan kromatografi lapis tipis dengan menggunakan beberapa jenis campuran eluen. Hasil dari penelitian ini adalah golongan senyawa metabolit sekunder yang terkandung dalam ekstrak etanol daun Calathea silver adalah golongan flavonoid, alkaloid, tanin, dan steroid. Berdasarkan profil kromatografinya terdapat 5 spot yang dapat terelusi dengan baik. Masing-masing spot memiliki nilai Rf yaitu, 0,4; 0,5; 0,7; 0,8 dan 0,9. Eluen yang baik dan yang dapat digunakan untuk proses pemisahan selanjutnya adalah eluen campuran kloroform : metanol dengan perbandingan 10:1.
Jamu pegal linu is one of the traditional medicinal products that are in great demand by the public, because it can relieve muscle and bone pain, improve blood circulation, strengthen body resistance, and relieve pain all over the body. However, some industry players have added medicinal medicine such as dexamethashone and mefenamic acid in herbal medicine. This study aims to determine the validity of the method in the analysis of dexamethashone and mefenamic acid by UV-Vis spectrophotometry on herbal medicine circulating in Indonesia several markets in Kediri-East Java. The sampling technique used in this study is a purposive sampling method so as to get 5 samples of herbal medicine (A,B,C,D,E). Research begins with determined of wavelength (nm) maximum of dexamethashone and mefenamic acid standard at 200-400 nm and determined of method validation to ensure the accuracy of the method in determining dexamethashone and mefenamic acid levels in the sample. The results of the research showed that the wavelength maximum of mefenamic acid and dexamethashone standard were 288 nm and 245 nm. The method validation showed that this method is good for detect the presence of mefenamic acid and dexamethasone in herbal medicine with the value of the validation parameters, such as linierity of calibration curve of mefenamic acid and dexamethasone weas 0.998, detection limit (LOD) 0.8779 µg/mL and 0.9677 µg/mL ; quantification limit (LOQ) 2.9264 µg/mL and 2.2256 µg/mL; intraday and interday precision of mefenamic acid was expressed by the value of % standard deviation relative (%RSD) was 0.8905 % and 1.0781 %; intraday and interday precision of dexamethasone was expressed by the value of % standard deviation relative (%RSD) was 0.6917 % and 0.8062 %. Respectively; as well as the accuracy expressed in mean % recovery were 101.547 % for mefenamic acid, and 100.576% for dexamethasone. Results analysis of the sample using a validated method showed that only sample A was mefenamic acid positively with concentration was 7.6796 µg/mL. The other hand, sample C,D and E was dexamethashone positively with concentrations were 2.4978 µg/mL, 2.4112 µg/mL and 8.7748 µg/mL.
Antioxidant compounds are able to dampen or ward off free radicals. Living things including pearl mussels (Pinctada maxima) can produce secondary metabolite compounds (phenolics, alkaloids, and terpenoids). There have been no studies on the antioxidant potential, flavonoid content, and total phenolics of pearl clam shells. This study aimed to determine the antioxidant potential of pearl clam shell ethanol extract (EECKM) in vitro using the DPPH method. In addition, it aims to determine the total flavonoid and phenolic levels of EECKM. The powder of pearl mussel shells is soaked with ethanol and the filtrate is concentrated until a concentrated extract is obtained. Flavonoid and phenolic levels from EECKM were measured using a UV-Vis spectrophotometer. Measurement of antioxidant activity using the DPPH method. EECKM has a yield of 1.73%, flavonoid levels of 0 mg QE/g, and total phenolics of 4.8 mg GAE/g. The extract has the highest antioxidant activity for a concentration of 100 mg / L with a percent value of DPPH radical inhibition of 67.1%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.